Combined Effect of Iodine Contrast Media, Cisplatin and External Beam Radiotherapy on Anaplastic Thyroid Cancer Cells
Journal Title: Journal of Biomedical Physics and Engineering - Year 2019, Vol 9, Issue 2
Abstract
Introduction: The current study investigated the combination of high Z atoms (iodine-, platinium-based drugs) with using low energy irradiation (120kvp) in Anaplastic Thyroid cancer cells. Material and Methods: For this purpose, eight groups were designed: control (CNT), different concentrations of Iodine contrast media (ICM), irradiation with various doses, Cis-platin (CDDP) with different concentrations, (ICM + CDDP), (ICM + RAD), (CDDP + RAD) and (ICM + CDDP + RAD). The viability was measured by MTT and Colony assay. In MTT assay, the viability of 8305c cells RAD (2 Gy)+ICM (10mg/mL) group was significantly lower than those treated with RAD or ICM alone. CDDP +ICM+RAD group significantly decreased the viability. In colony assay, cells in ICM + RAD (2 Gy) group reduced the number of colonies more significant than RAD group. The difference of colony forming ability between CDDP and CDDP + RAD (2 Gy) was significant. The difference of ICM + CDDP + RAD (2 Gy) and CDDP +RAD (2 Gy) group was significant. All data were statistically analysed using one-way analysis of variance (ANOVA) followed by Chafe’s multi-comparisons tests. All data were presented as mean ± standard deviation (SD) and analysed using statistical package for social sciences (SPSS 16). Significance was considered to be p<0.05. Results: In MTT assay, the viability of 8305c cells RAD (2 Gy) + ICM (10mg/ mL) group was significantly lower than those treated with RAD or ICM alone. CDDP + ICM + RAD group significantly decreased the viability. In colony assay, cells in ICM + RAD (2 Gy) group reduced the number of colonies more significantly than RAD group. The difference of colony forming ability between CDDP and CDDP + RAD (2 Gy) was significant. The difference of ICM + CDDP + RAD (2 Gy) and CDDP + RAD (2 Gy) group was significant. Conclusion: Exposure of ATC to ICM in the presence of CDDP increases tissue X-rays absorbance by Auger electrons and photo electrons leading to more fatal effects against the tumour.
Authors and Affiliations
A Shabestani Monfared, S. Nadi, E. Zabihi, A. Mahmoudzadeh, N. Eyvazzadeh, R. Tahamtan
Keywords
Related Articles
- EP ID EP613514
- DOI -
- Views 116
- Downloads 0
Monte Carlo Simulation of Siemens Primus plus Linac for 6 and 18 MV Photon Beams
Objective: The aim of the present study is to simulate 6 MV and 18 MV photon beam energies of a Siemens Primus Plus medical linear accelerator (Linac) and to verify the simulation by comparing the results with the measur...
Dosimetry and Radioenhancement Comparison of Gold Nanoparticles in Kilovoltage and Megavoltage Radiotherapy using MAGAT Polymer Gel Dosimeter
Background: Numerous unique characteristics of the nanosized gold, including high atomic number, low toxicity, and high biocompatibility make it one of the most appropriate nanostructures to boost radiotherapy efficacy....
Dosimetry of Critical Organs in Maxillofacial Imaging with Cone-beam Computed Tomography
Background: While the benefits of cone-beam computed tomography (CBCT) are well known in maxillofacial imaging, the use of this modality is not risk-free. Objective: The aim of this study was to evaluate the exposure dos...
Evaluation of Gold Nanoparticle Size Effect on Dose Enhancement Factor in Megavoltage Beam Radiotherapy Using MAGICA Polymer Gel Dosimeter
Background: Gold nanoparticles (GNPs) are among the most promising radiosensitive materials in radiotherapy. Studying the effective sensitizing factors such as nanoparticle size, concentration, surface features, radiatio...
Radiation-induced Non-targeted Effect and Carcinogenesis; Implications in Clinical Radiotherapy
Bystander or non-targeted effect is known to be an interesting phenomenon in radiobiology. The genetic consequences of bystander effect on non-irradiated cells have shown that this phenomenon can be considered as one of...