Comet assay of infestation degree of carp yearlings blood lymphocytes DNA under the effect of ectoparasites
Journal Title: Біологія тварин - Year 2014, Vol 16, Issue 2
Abstract
The results of research of infestation degree of carp yearlings blood lymphocytes DNA under the invasion by ectoparasites Lernaea cyprinacea and Dactylogyrus vastator and under the mixed infestation are presented in the article. Mutagenic properties of ectoparasite infestation on the host body are proved by study results of its genotoxic and cytotoxic influence on the basis of modern DNA-comet assay on the cells of carp blood lymphocytes. To test this hypothesis the research of fragmented DNA level in carp yearlings lymphocytes under the influence of ectoparasites had been conducted using the DNA-comet method. For this purpose twelve groups were formed with 6 individuals in each with varying degrees of infestation. They were presented by four groups of fish (control and three experimental) under the infestation by ectoparasites Lernaea cyprinacea, Dactylogyrus vastator and under the mixed infestations. Under the invasion by Lernaea cyprinacea the blood lymphocytes apoptotic cells of the fishes of 3rd experimental group exceeded the control 1.2 times (P<0.05). Under the invasion >0.26 ind./g b.w. (4th experimental group) the percentage of “tail moment” was 3.4 times higher than of control one (P<0.01). The number of apoptotic cells exceeded 1.2 times this amount in the blood lymphocytes of control fish (P<0.01). Under the invasion of fish by Dactylogyrus vastator reliable values of the “tail moment” and apoptotic cells in blood lymphocytes were registered in the fish of 4th experimental group, namely 2.6 (P<0.05) and 1.3 times (P<0.05), respectively. When researching “tail moment” index in lymphocyte cells under the mixed invasion it was set up its reliable growth in 2nd, 3rd and 4th experimental groups, respectively, 2.3 times (P<0.05), 4.3 (P<0.01) and 4.8 times (P<0.001). However, it was registered the reliable increasing of the apoptotic cells number in blood lymphocytes of the fishes of 3rd and 4th experimental groups in 1.6 (P<0.05) and 1.9 times (P<0.01). There were observed in our study a certain regularity of ectoparasites effect on fish blood cells, which was characterized by increasing of “tail moment” index and the number of apoptotic cells along with increasing of infestation degree. Apparently, ectoparasites have genotoxic and cytotoxic effect on fish blood cells, cause the growth of single-stranded breaks of alkali labile sites of nuclear DNA molecule of apoptotic cells in blood lymphocytes, which is directly proportional to the increase in the number of ectoparasites.
Authors and Affiliations
Yu. V. Loboyko, V. V. Stybel
Keywords
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