COMPARATIVE ANALYSIS OF BIOLOGICAL ACTIVITY OF SILYBUM MARIANUM L. FOOD SUPPLEMENTS AVAILABLE ON MARKET: INVITRO STUDY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 11
Abstract
Objective: Silybum marianum L. Food Supplements that contain silymarin is widely used as a therapeutic agent in liver diseases. Many brands are available on the market in USA, Egypt, Europe and other countries. The objective of this study was to compare the biological activity in different preparations of silymarin available on the market in USA and Egypt using paracetamol-induced oxidative stress injury on primary cultured rat hepatocytes.Methods: Forty four silymarin samples available on the market were collected from USA (24) and Egypt (20) and tested for hepat protective antioxidant effects on primary cultured rat hepatocytes. Cytotoxicity was measured by MTT [3-(4, 5-dimethyl-thiazol-2)-2,5-diphenyl tetrazolium bromide] assay and lactate dehydrogenase (LD) leakage into culture medium. Antioxidant effects were determined by glutathione reductase (GR), and Nitric oxide (NO) assays in silymarin, pretreated rat hepatocytes for 2 h followed by incubation with 25 mM paracetamol over a period of 1 h. Therapeutic index was calculated for each tested sample for comparative analysis.Results: Silymarin preparations significantly decreased toxicity induced by paracetamol in rat hepatocytes, decreased lactate dehydrogenase leakage and prevented GSH depletion (P<0.01) and returned NO to basal levels in rat hepatocytes. The therapeutic index was 80, 40 and 20 for samples No. 20, 19 and 5 respectively.Conclusions: The 44 different silymarin preparations tested in this study exhibited variation in antioxidant capacity and in reducing nitric oxide produced as a result of paracetamol injury. This variation in biological activity did not always correspond to the amount of silymarin recorded on samples.Â
Authors and Affiliations
Heba D. Hassanein, Mona M. Abdel Mohsen, Khaled A. Shams, Nahla S. Abdel-azim, Mahmoud A. Saleh, Nermine A. Ehsan, Faiza M. Hammouda
Keywords
Related Articles
- EP ID EP578127
- DOI -
- Views 47
- Downloads 0
NOVEL HPLC-UV METHOD USING VOLATILE BUFFER FOR SIMULTANEOUS DETERMINATION OF AMLODIPINE BESYLATE AND ATORVASTATIN CALCIUM
Objective: The purpose of this work was to develop and validate a novel HPLC-UV method using triethylamine (TEA) as a volatile buffer for simultaneous determination of amlodipine besylate (AML) and atorvastatin calcium (...
GARDINERIN, A BIOLOGICALLY ACTIVE ACETOGENIN FROM THE SRI LANKAN GONIOTHALAMUS GARDINERI HOOK. F. AND THOMSON
Objective: The study was undertaken to isolate biologically active compounds from Goniothalamus gardineri, a plant endemic to Sri Lanka. Methods: Roots and flowers of Goniothalamus gardineri were extracted into dichlorom...
ANTIHYPERGLYCEMIC ACTIVITY OF PHENYL AND ORTHO-HYDROXY PHENYL LINKED IMIDAZOLYL TRIAZOLO HYDROXAMIC ACID DERIVATIVES
Objective: The paradigm was to establish phenyl and ortho-hydroxy phenyl linked imidazolyl triazolo hydroxamic acid derivatives as an antihyperglycemic agent.Methods: 100 mg/Kg body weight dose of phenyl and ortho-hydrox...
QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP ANALYSIS OF NOVEL PYRAZOLINE DERIVATIVES USING K NEAREST NEIGHBOUR MOLECULAR FIELD ANALYSIS METHOD
Objective: Malonyl CoA decarboxylase (MCD) enzyme plays important role in fatty acid and glucose oxidation. Inhibition of MCD might turn to a novel approach to treat ischemia. The main objective of this research article...
STANDARDIZATION OF FRIABLE CALLUS DEVELOPMENT IN CATHARANTHUS ROSEUS (LINN.) G. DON
Objective: The objective of the study was to develop an effective hormonal combination for the maximum growth of callus and development of friable calli using the same medium with reduced concentration of agar.Methods: T...