Comparative characteristics of proteolytic activity in case of experimental peritonitis and its development on the background of diabetes mellitus
Journal Title: Міжнародний ендокринологічний журнал - Year 2019, Vol 15, Issue 3
Abstract
Background. The relevance is due to rather understudied state of proteolytic reactions in case of diabetes mellitus (DM) with acute peritonitis (AP), which is increasingly common in the practice. Objective: to study the features of proteolytic activity of plasma in AP associated with DM. Materials and methods. One hundred albino outbred rats. AP was simulated by the transesophageal perforation of the stomach. DM was simulated by the 1.6% alloxan solution injection. The proteolytic activity of blood plasma was studied by azocasein (AzCs), azoalbumin (AzAl), and azocollagen (AzCl). The animals were divided into the following groups: intact rats, animals with simulated DM, intact rats with simulated peritonitis (group 1), animals with models of peritonitis on the background of DM (group 2). Results. The initial level of proteolytic transformation of AzCs and AzAl in animals with simulated DM was significantly higher. The proteolytic transformation level of AzCl had almost no differences. Six hours after the moment AP was modeled, the proteolytic transformation level of AzCs increased in both groups, more significantly in group 1, although this indicator in group 1 remained less. The proteolytic transformation level of AzAl increased significantly. The proteolytic transformation level of AzCl in group 1 remained almost the same, and increased significantly in group 2. In 12 hours, the proteolytic transformation level of AzCs decreased slightly in group 1 but continued to increase in group 2. The proteolytic transformation level of AzAl significantly increased in both groups, and the indicators in group 2 were higher. The proteolytic transformation level of AzCl decreased statistically significantly in group 1, and greatly increased in group 2. In 24 hours, the proteolytic transformation level of AzCs had almost no changes in group 1 but continued to increase in group 2. The proteolytic transformation level of AzAl decreased significantly in group 1 and greatly increased in group 2. The proteolytic transformation level of AzCl significantly increased in both groups. In 48 hours, the proteolytic transformation level of AzCs and AzAl increased in both groups but the indicators in group 2 were higher. The proteolytic transformation level of AzCl decreased slightly in group 1, and continued to increase in group 2. Conclusions. The proteolytic transformation activity of high and low molecular weight blood plasma proteins increases in experimental diabetes mellitus. The proteolytic system of plasma is activated, with maintaining the balance between its links within 24 hours in experimental acute peritonitis. The development of acute peritonitis in animals with simulated diabetes mellitus differs greatly in 6 hours by quantitative characteristics of the proteolytic activity of blood plasma that manifested with its significant increase, some imbalance between the links of proteolysis with the signs of uncontrolled proteolysis in 24 hours. The differences being detected are due to the changes in the functional activity of the proteolytic system caused by diabetes mellitus that underlies the disorders of the mechanisms of inflammation regulation.
Authors and Affiliations
F. V. Grynchuk, A. F. Grynchuk
Keywords
Related Articles
- EP ID EP605788
- DOI 10.22141/2224-0721.15.3.2019.172104
- Views 50
- Downloads 0
Features of Type 2 Diabetes Mellitus in Combination with Hypothyroidism
Background. The last decades are characterized by the considerable increase in the prevalence of endocrine disorders with the change of the structure, and first of all cases of polyendocrinopathy, the special place among...
Diabetic Retinopathy: a New Approach to Drug Treatment
Diabetic retinopathy is a basic microvascular complication of diabetes and the leading cause of blindness in the adult population. A review of the literature on this pathology, a relatively new approach to medical treatm...
Features of Neuroendocrine and Hormonal Disorders at Growth Hormone Deficiency in Adults with Mass Lesions of Hypothalamus-Pituitary Region
In this article the author analyzes the status of 40 adults with tumors of the pituitary region, of whom 35 (87.5 %) had growth hormone deficiency with neuroendocrine complications. Specific for growth hormone deficiency...
Influence of Overweight and Obesity on Reproductive Health and Carrying of a Pregnancy
The lecture considers the influence of obesity in women of reproductive age on somatic and reproductive problems that results in the decline of fertility. Women with obesity in comparison to the women with normal body we...
Normogonadotropic ovarian insufficiency in adolescence (review of literature and clinical case)
The objective of the review. Analysis of literature data related to the normogonadotropic ovarian insufficiency. Basic statements. The article presents a literature review and clinical case of normogonadotropic ovarian i...