Comparative study of antibacterial activity of two different earthworm species, Perionyx excavatus and Pheretima posthuma against pathogenic bacteria
Journal Title: Journal of Applied and Natural Science - Year 2015, Vol 7, Issue 2
Abstract
Disease outbreaks are being increasingly recognized as a significant constraint on aquaculture production and trade affecting the economic development of the sector in many countries. Extracting and using biologically active compounds from earthworms has traditionally been practiced by indigenous people throughout the world. The aim of the present study was to shown antimicrobial activity through earthworm extract against fish bacterial pathogens. In total, 8 bacterial strains i.e. 6 gram negative viz. Aeromonas hydrophila, Pseudomonas aeruginosa, P. fluorescens, E.coli, Enterobacter aerogens and Shigella sp. and 2 gram positive viz. Staphylococcus aureus and Micrococcus luteus were identified. The extract of earthworm Perionyx excavatus, Pheretima posthuma were prepared and antimicrobial activity of the extract was determined by antimicrobial well diffusion assay. After 24 hrs of incubation period, it was observed that earthworm extract showed antibacterial activity against isolated bacterial strains. Among earthworm extract of two different species, the maximum zone of inhibition was shown against A. hydrophila by Perionyx excavatus (18.33± 0.66 mm) and P. posthuma (16.66±0.33). P. excavatus showed antibacterial activity against all pathogenic bacteria except Shigella spp. However on the other hand, P.posthuma showed antibacterial activity against A. hydrophila, P. fluorescens, E.coli, and S. aureus. The study has proved that earthworm extract can be effectively used for suppression of bacterial infection in fishes and that it can used as potential antimicrobial drug against commercial antibiotic resistance bacteria.
Authors and Affiliations
Nitish Bansal, R. K. Gupta, Dharambir Singh, Shashank Shashank
Keywords
Related Articles
- EP ID EP283360
- DOI 10.31018/jans.v7i2.664
- Views 72
- Downloads 0
Heterosis, dominance estimate and genetic control of yield and post harvest quality traits of tomato
Paucity of research on the development of tomato hybrid having desirable post harvest/or processing quality in the tropics compel to undertake this study. Therefore, the present investigation was undertaken to identify p...
Genetic variability, heritability and genetic advance for various quantitative and qualitative traits in Chilli (Capsicum annuum L.)
Genetic variability, heritability and genetic advance for different quantitative and qualitative characters were studied in 64 genotypes of chilli. The study indicated the existence of considerable amount of genetic vari...
Influence of sowing dates and nitrogen levels on growth, yield and quality of scented rice cv. Pusa Sugandh-3 in Kashmir valley
A field experiment was carried out to determine the optimum sowing date and nitrogen (N) level for the scented rice cv. Pusa Sugandh-3. Twelve treatment combinations of 3 sowing dates, viz., 15th, 16th and 18th standard...
Productivity, profitability and water use efficiency of wheat (Triticum aestivum L.) under different customized fertilizers and moisture regimes
A field experiment was conduct during the winter (rabi) season of 2010-2011 on silty loam soil at Faizabad (Uttar Pradesh), India to develop suitable dose of customized fertilizers (CF) and moisture regime for wheat (Tri...
Semi-dwarf narrow rolled leaf mutant in rice: Photosynthetic efficiency and physiological response to gibberellic acid (GA3)
Leaf is the major component for fixation of carbondioxide to produce more photoassimilates and maintains source sink relationship in plants. The present investigation was carried out to study the photosynthetic efficienc...