Comparative Study of DPPH, ABTS and FRAP Assays for Determination of Antioxidant Activity
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2015, Vol 3, Issue 6
Abstract
Three simple spectrophotometric methods: (1, 1-diphenyl-2-picrylhydrazyl) free radical scavenging activity assay (DPPH), [2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid)] free radical scavenging activity assay (ABTS) and Ferric ion Reducing Antioxidant Power assay (FRAP) for determination of antioxidant activity were compared. The antioxidant activity of aqueous extracts of the mycelia of three mushroom species (Ganoderma lucidum, Lentinus edodes and Agaricus bisporus) were compared for this study. A comparative account was made between the three antioxidant assays and their advantages and disadvantages were briefly reported along with the scope of application of each method. DPPH was found to be the most preferred method for the determination of antioxidant activity of mycelia of the three mushroom species because it could be rapidly performed and showed high reproducibility. Results were reported so that it becomes easy to select the right method that can give accurate results for the antioxidants in study.
Authors and Affiliations
Pooja Shah, H. A. Modi
Keywords
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