COMPARATIVE STUDY OF IMMUNOLOGICAL METHODS FOR THE DIAGNOSIS OF CHLAMYDIA TRACHOMATIS IN WOMEN WITH CERVICITIS AND INFERTILITY.
Journal Title: International Journal of Advanced Research (IJAR) - Year 2019, Vol 7, Issue 1
Abstract
We have evaluated the diagnostic accuracy of three ELISA tests (IgM, IgG, IgA) and rapid test in detecting C. trachomatisby comparing with direct immunofluorescence (DIF), which is used as a gold standard. Blood samples and endocervical swabs of 100suspectedfemales and 10 healthy women were collected. All sera were tested for chlamydial antibodies of the IgG, IgM and IgA classes by ELISA. Swabs were tested by Rapid test and DIFfor detection of C. trachomatis antigen. Among 100 patients that were included in the study, 46 (46%) women with infertility and 4 (4%) with cervicitis were positive for chlamydia according to the standard method (DIF).None of the healthy women was positive. When compared to DIF results; the ELISA (IgM, IgG, IgA) and rapid test results showed noteworthy differences in the major performance characteristics. The sensitivity of IgM, IgA, IgG and Rapid test was(66, 62, 48, and 24%),specificity (96, 96, 92, and 94%), positive predictive values (94.3, 93.3, 58.7, and 80%),negative predictive values (73.8, 71.6, 63.9, and 55.3%)and Area Under a Curve (0.81, 0.81, 0.7, 0.59.3%) respectively.The results concluded that DIF, IgM, and IgA are valuable techniques to diagnose chlamydial infections. On the other hand, under the conditions of our study, the results suggested that IgG and rapid test are not reliable for diagnosis of chlamydial infections.
Authors and Affiliations
Abrar A. H. Sahi, Khairallah A. S. Mohammed, Zainab Y. Al Salman.
Keywords
Related Articles
- EP ID EP459241
- DOI 10.21474/IJAR01/8358
- Views 79
- Downloads 0
THE TOURISM SECTOR, ITS IMPORTANCE IN ACHIEVING ECONOMIC GROWTH IN THE SULTANATE OF OMAN.
This paper focuses on the importance of Tourism Sector for a country\'s economy. In order to conduct this research paper, Oman has been chosen as the country. The aim of this paper is to identify how Oman\'s tourism indu...
A COMPARATIVE STUDY OF EFFICACY OF INTRATHECAL BUPIVACAINE AND LEVOBUPIVACAINE FOR CAESAREAN SECTION.
Introduction: Spinal anaesthesia is a balance technique used for cesarean delivery. It provides rapid onset of anesthesia and complete muscle relaxation. Objective: To assess and compare the onset of sensory and motor bl...
WATER POLLUTION AT MIRIK LAKE IN DARJEELING, WEST BENGAL: A LOCAL SOCIOECONOMIC IMPACT ANALYSIS.
Mirik Lake is one of the popular tourist destinations in the Darjeeling mountain area of West Bengal, India. Due to the large number of tourists? inflow in Mirik Lake region, the demand for tourism and hotel industry has...
ERBIUM LASERS IN PERI IMPLANTITIS ? A REVIEW.
Dental implants are a well-established and reliable option for the treatment of complete and partial edentulism and has been associated with high survival rates both in immaculate and augmented/regenerated bone. Unforese...
AWARENESS, PERCEPTIONS AND KNOWLEDGE OF AMBLYOPIA AMONG PEDIATRICS AND OPHTHALMOLOGY CLINICS ATTENDEES IN KING ABDULAZIZ UNIVERSITY HOSPITAL, JEDDAH.
Objectives: To assess the knowledge, awareness, and perception about amblyopia and related disease dimensions among the parents and companions of children attending the pediatrics and ophthalmology clinic. Methodology: A...