COMPARATIVE STUDY OF THE ANTIMICROBIAL, MINIMUM INHIBITORY CONCENTRATIONS (MIC), CYTOTOXIC AND ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF DIFFERENT PARTS OF PHYLLANTHUS ACIDUS (L.) SKEELS (FAMILY: EUPHORBIACEAE)
Journal Title: WORLD JOURNAL PHARMACY AND PHARMACEUTICAL SCIENCE - Year 2019, Vol 8, Issue 1
Abstract
The main aim of this study was to find out and compare the antimicrobial, cytotoxic, antioxidant activity along with the minimum inhibitory concentrations (MIC) of methanolic extract of different parts of Phyllanthus acidus (L.) skeels (family: Euphorbiaceae). The methanolic extract of Phyllanthus acidus seed and fruit had shown average activity of (20-24.5) mm and (20-23.5) mm accordingly. The maximum zone of inhibition for seed extract was 24.5mm, 20mm against Staphylococcus aureus,Bacillus subtilis and Salmonella typhi. Using the fruit extract it was 23.5mm, 20mm against Vibrio mimicus, Sarcina lutea and Salmonella typhi. The leaf, bark and root extracts of the plant had shown average activity of (9-16), (9-17) and (5-9.5) mm respectively while the root part showed lowest antimicrobial activity of (5-7) mm. The minimum inhibitory concentration (MIC) of seed extract for Vibrio mimicus and Staphylococcus aureus were 8μg and 32μg respectively while (MIC) of fruit extract for Vibrio mimicus was 2μg and 16μg against Staphylococcus aureus. In comparison, the best MIC was shown by fruit part (2μg) against Vibrio mimicus. The Brine Shrimp Lethality Bioassay method was used to determine the cytotoxicity activities of seed, root, fruit, bark and leaf part whose LC50 value was 17.09 μg/ml,51.56 μg/ml,18.89 μg/ml, 25.03 μg/ml, and 35.71 μg/ml respectively. The IC50 value of ascorbic acid was used as reference standard obtained 43.27 μg/ml. The seed, fruit and root partThe main aim of this study was to find out and compare the antimicrobial, cytotoxic, antioxidant activity along with the minimum inhibitory concentrations (MIC) of methanolic extract of different parts of Phyllanthus acidus (L.) skeels (family: Euphorbiaceae). The methanolic extract of Phyllanthus acidus seed and fruit had shown average activity of (20-24.5) mm and (20-23.5) mm accordingly. The maximum zone of inhibition for seed extract was 24.5mm, 20mm against Staphylococcus aureus,Bacillus subtilis and Salmonella typhi. Using the fruit extract it was 23.5mm, 20mm against Vibrio mimicus, Sarcina lutea and Salmonella typhi. The leaf, bark and root extracts of the plant had shown average activity of (9-16), (9-17) and (5-9.5) mm respectively while the root part showed lowest antimicrobial activity of (5-7) mm. The minimum inhibitory concentration (MIC) of seed extract for Vibrio mimicus and Staphylococcus aureus were 8μg and 32μg respectively while (MIC) of fruit extract for Vibrio mimicus was 2μg and 16μg against Staphylococcus aureus. In comparison, the best MIC was shown by fruit part (2μg) against Vibrio mimicus. The Brine Shrimp Lethality Bioassay method was used to determine the cytotoxicity activities of seed, root, fruit, bark and leaf part whose LC50 value was 17.09 μg/ml,51.56 μg/ml,18.89 μg/ml, 25.03 μg/ml, and 35.71 μg/ml respectively. The IC50 value of ascorbic acid was used as reference standard obtained 43.27 μg/ml. The seed, fruit and root part showed high to moderate antioxidant potential with IC50 of 38.17 μg/ml,43.01 μg/ml and 54.57 μg/ml respectively. The amount of total phenolic content varied and ranged from 18.36 to 30.77 mg of GAE/gm of extractives. The highest phenolic content was found in seed (30.77 mg of GAE/gm of extractives) while fruit contained (30.03 mg of GAE/gm of extractives).
Authors and Affiliations
Tanvir Ahmed
Keywords
Related Articles
- EP ID EP666812
- DOI -
- Views 126
- Downloads 0
ASSESSMENT OF ACUTE AND SUBACUTE TOXICITY OF AQUEOUS EXTRACT AND IN VITRO SUSCEPTIBLITY OF EXTRACTS AND ISOLATED INDOLOQUINOLINE ALKALOIDS FROM CRYPTOLEPIS SANGUINOLENTA (LINDL.) SCHLECHTER (PERIPLOCACEAE) ROOT BARK TO ENTAMOEBA HISTOLYTICA
Aqueous and 80% methanol extracts from Cryptolepis sanguinolenta root bark and their alkaloids were submitted to an evaluation for their potential amoebicidal activity against Entamoeba histolytica. Results indicated tha...
REVIEW ON BUCCUL PATCHES AS A NOVEL DRUG DELEVERY SYSTEM
Buccal drug delivery systems relate with the mucus layer casing the mucosal epithelial superficial, and mucin molecules and rise the residence time of the dosage form at the location of absorption. The objective of this...
COMPARISON BETWEEN PHENOTYPIC AND GENOTYPIC METHODS FOR THE DETECTION OF METALLO BETA LACTAMASES PRODUCING PSEUDOMONAS AERUGINOSA
Background: Pseudomonas aeruginosa is a frequent nosocomial pathogen that causes severe diseases in many settings. Carbapenem resistance may be conferred through various mechanisms, including the expression of carbapenem...
INVESTIGATION OF ANTI-OBESITY ACTIVITY OF ETHANOLIC EXTRACT OF FOENICULUM VULGARE SEEDS, IN VIVO AND IN SILICO MODELS
Background: Obesity is a chronic disease that triggers metabolic disorders due to excessive internal fat accumulation. Foeniculum vulgare (FV) is a very popular medicinal plant used to control obesity in the traditional...
REVIEW ON EPISIOTOMY WOUND CARE - AN INSIGHT THROUGH AYURVEDA
Episiotomy is given during vaginal delivery to prevent the pelvic floor laceration. Approximately 70% of women who had vaginal delivery will experience some degree of injury to perineum, due to perineal tear or episiotom...