Comparison of antiaspergillar activity of extracts of Tectona grandis Linn according to two antifungal susceptibility testing
Journal Title: UNKNOWN - Year 2016, Vol 5, Issue 3
Abstract
Plants used as antimicrobials in the treatment of infectious diseases in folk medicine are regularly evaluated in different Laboratories. And, the processes of evaluation of antifungal susceptibility are often different to one laboratory to another. This study was undertaken in the goal to compare parameters obtained (MIC and MFC) with two antifungal susceptibility testing. The first one is Agar slant double dilution tubes method (M1) and the second is disc diffusion method /broth dilution according document CLSI M38 -A2 supplemented to 2% glucose (M2). Ethanol (EtOH 70%), Methanol (MeOH) and n-Hexane (n-HexTG) extracts of leaves of Tectona grandis have been tested in comparison to Itraconazole against two clinicals strains of Aspergillus fumigatus 896/AB and Aspergillus flavus 1006/OM isolated to HIV patients. Results showed that on A. flavus, agar slant method (M1) gave MIC ranging between 200 µg/mL and 800 µg/mL while disc diffusion meth/broth dilution (M2) showed growth inhibition between 100 µg/mL and 400 µg/mL. On A. fumigatus, inhibition was observed between 200µg/mL and 400 µg/mL with M2 in contrary M1 gave MIC located between 400 µg/mL and 800 µg/mL. For each strain tested, according method M2, MIC and MFC are identical and with method M1, these parameters were different. Evaluation of Itraconazole on each strain gave with M2, MIC= 50 µg/mL while inhibition has been showed at 100 µg/mL and 200µg/mL with M1. Also considering extracts, hydroalcoholic extract of T. grandis (EtOHTG) showed antifungal susceptibility testing less 2 to 4 efficiency than Itraconazole. Disc diffusion method/broth dilution (M2) according CLSI M38-A2 to evaluate Tectona grandis antifungal susceptibility is the process of evaluation which allo wed having lo west antifungal parameters (MIC and MFC) on A. flavus and A. fumigatus.
Authors and Affiliations
Elisee Kporou Kouassi, Ibourahema Coulibaly, Gervais Melaine M'boh, Ouattara Sitapha, Mathieu Kra Adou Koffi, Ilioara Oniga, Joseph Djaman Allico
Keywords
Related Articles
- EP ID EP455376
- DOI -
- Views 92
- Downloads 0
Antioxidant and antidiabetic activity of Phoenix pusilla Gaertn. unripe fruit extract in streptozotocin-induced sprague dawley rats
The fruit of Phoenix pusilla Garetn. has been used in herbal medicines, as it is sweet, sour, cooling and laxative, cardiotonic, aphrodisiac, carminative and roborant. The objective of the present study was to investigat...
Effect of Annona senegalensis leaves on morphometric parameters of reproductive organs in rabbits
The present study aimed to evaluate the effect of the leaves of Annona senegalensis on morphometric parameters of the female reproductive organs in rabbits. A total of 120 rabbits were divided into four batches A, B, C a...
Variation in the origin of left testicular artery and drainage of right testicular vein
During routine dissection of 65 year old male Indian cadaver posterior abdominal wall, variations in the testicular vessels were observed. The left testicular artery arose from the left accessory renal artery, which orig...
Acute oral toxicity study of Tectona grandis LInn. methanolic seed extract in albino mice
Objective: To evaluate the acute toxicity studies of methanolic seed extract of Tectona grandis in albino mice. Method: The present study has been carried out to evaluate the adverse and hazardous effects of methanolic s...
Ethnomedicinal, pharmacological and antimicrobial aspects of Moringa oleifera Lam.: A review
The inclusive information is provided in present review on traditional uses, antimicrobial activity and pharmacology of Moringa oleifera Lam. It is commonly known as ‘drumstick tree’. M. oleifera is alternative tonic, as...