Comparison of hipO and ceuE Gene Based PCR Assays for the Detection of Campylobacter Jejuni
Journal Title: Journal of Clinical Microbiology and Biochemical Technology - Year 2016, Vol 2, Issue 1
Abstract
The objective of this study was to find out the reproducibility and specifity of hipO and ceuE genes based PCR assays for the detection of Campylobacter jejuni isolated from turkey meat samples in a previous study. A total of 44 Campylobacter isolates including 41 C. jejuni, two C. coli and one C. lari were used in this study. Although all of the C. jejuni isolates were verified by hipO based PCR assay, only 18 of the 41 C. jejuni were detected as positive by ceuE based PCR assay. Both of the methods showed negative reaction with C. coli and C. lari isolates. The results showed that, hipO gene based PCR assay is more reproducibly and specific than ceuE gene specific PCR analyze for the detection and identification of C. jejuni.
Authors and Affiliations
Ayaz Naim Deniz, Goncuoglu Muammer, Cakmak Omer, Erol Irfan
Keywords
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- EP ID EP353342
- DOI 10.17352/2581-527X.000008
- Views 100
- Downloads 0
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