Comparison of Platelet-Rich Plasma (PRP), Bone Marrow-Derived Mesenchymal Stem Cells and their Combination on the Healing of Achilles Tendon in Rabbits
Journal Title: Iranian Journal of Veterinary Surgery - Year 2017, Vol 12, Issue 2
Abstract
Objectives- The purpose of the study was to observe whether PRP, Mesenchymal Stem Cells (MSCs) and combining them could help make improvements the healing of Achilles tendons in rabbits. Design- Experimental study. Animals-Sixteen male, healthy and mature white New Zealand rabbits. Procedures- The animals were randomiaed into 4 groups: Control, PRP, MSCs with fibrin glue and MSCs + PRP therapy. A 3 mm diameter defect was created in the midsubstance of the medial M. gastrocnemius tendon in all rabbits. After 4 and 8 weeks, animals were euthanized (each time point, 2 rabbits of the each group) and samples were examined in term of histologic scoring with H & E and Masson's Trichrome. Results- Histologic investigations confirmed that all 3 methods of treatment in Achilles tendon defect had suitable reparative effects. Final histologic scoring for control group at 4th week after operation was 4.5 in contrast to treatment groups (PRP =6, MSCs = 5.5 and MSCs + PRP= 8). Final histologic scoring in all 4 groups improved at 8th week after operation (control=6.5, PRP =9.5, MSCs = 8.5 and MSCs + PRP= 14.5). Conclusion and Clinical Relevance- This study indicated that using PRP or MSCs treatment alone and combination of them in injured tendon improved healing process and decreased time of repair. Reparative effect of PRP in tendon defect was better than use of allogenic MSCs treatment. However, the combination of them caused the best results.
Authors and Affiliations
Farnoosh Bagheri, Abbas Tavasoly, Mohammad Mehdi Dehghan, Farhang Sasani, Mina Raji, Saeed Farzad Mohajeri
Keywords
Related Articles
- EP ID EP633137
- DOI 10.22034/ivsa.2018.94255.1121
- Views 144
- Downloads 0
Immature Beluga (Huso huso); Heart; Anatomy; Echocardiography
Objective- The aim of this study was to obtain first time diagnosis of pregnancy and study of fetal development in different times of pregnancy period. Design- ِDescriptive study. Animals- 8 pregnant Markhoz goats. Proce...
Removing of Crop Foreign Body in a Cockatiel (Nymphicus hollandicus) by Ingluviotomy Technique: Case Report
Case description- A two-month-old cockatiel (Nymphicus hollandicus) of unknown sex ،weighing 58 g was referred for history of ingestion of foreign body inaction. Clinical findings- Of course, by examination and observati...
Evaluation of Pharmacological Effective Concentrations of Aspirin with/without Radiotherapy on MCF-7Breast Cancer Cell Line
Objective- The studies have been shown that aspirin, an anti-inflammatory agent, could reduce occurrence of different cancers. The aim of the present study was to evaluate pharmacological effective concentrations of aspi...
A Survey on Electrocardiographic Parameters in Broiler Chickens Following the Intracerebroventricular Injection of Melatonin
Objective- The pineal gland and its principal hormone melatonin play an important role in the circadian organization in birds. However, little is known about the role of melatonin in cardiac activity of broiler chicken....
Management of Large Chronic Non-healing Wounds by Autogenous Platelet Rich Plasma and Reconstructive Surgery in Three Cats
Case Description- Three domestic short haired cats (2, 5 and 4 years of age) were presented to the Madras Veterinary College Teaching Hospital, with the history of large chronic, full thickness wounds on the right latera...