COMPARISON OF PROPAGATION METHOD IN IN VITRO AND IN VIVO CONDITION OF Lonicera caerulea L.
Journal Title: Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura Alimentaria Piscaria et Zootechnica - Year 2017, Vol 42, Issue 334
Abstract
The aim of the study was to determine the effectiveness of rooting of blue honeysuckle of ‘Wojtek’ cultivar in vitro. Moreover, suitability of different soil substrate for rooting the lignified herbaceous cuttings in vivo was evaluated. The process of disinfection was the decisive factor in the process of preparing the explants for the propagation. Among the disinfection solutions used for sterilization of ‘Wojtek’ microshoots, the best results were obtained after application of 10% NaOCl solution. Initiation and propagation of blue honeysuckle proceed with the highest efficiency in Murashige and Skoog (MS) medium, and no positive effect of BAP cytokine on this process was reported. Proper selection of composition of the medium guaranteed high performance of rooting of explants in vitro – lack of plant growth regulators (PGRs) in MS medium led to a decrease in the percentage of rooted plants. The addition of auxins to MS medium had a stimulating effect on plant height, length, and number of roots. In the greenhouse, four-node lignified cuttings in the medium prepared with perlite and peat were rooted with the highest performance. Rooting of lignified herbaceous cuttings in the substrate prepared with peat and perlite stimulated the development of the root system as well. The worst influence for rooted cuttings was observed in sand.
Authors and Affiliations
Marcelina KRUPA-MAŁKIEWICZ, Ireneusz OCHMIAN, Miłosz SMOLIK, Krystyna Maria OSTROWSKA
Keywords
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