Comparison of Qiagen and Iontek Hepatitis B Virus-DNA Polymerase Chain Reaction Quantitation Kits in Chronic Hepatitis B Patients Infected with Hepatitis B Virus Genotype D
Journal Title: Viral Hepatitis Journal - Year 2020, Vol 26, Issue 1
Abstract
Objectives: Hepatitis B virus (HBV)-DNA level is a good marker for viral replication and should be monitored at regular intervals in patients with chronic hepatitis B (CHB). The objective of this study is to compare the performance of Qiagen HBV-DNA kit with Iontek HBV-DNA kit and to determine the usability of Iontek. Materials and Methods: Serum samples of 87 patients who had been identified as HBV genotype D previously, were sent to Kocaeli University for HBV-DNA quantitation. Serum HBV-DNA levels were determined by real-time polymerase chain reaction method using both systems simultaneously. The calculated viral load values were converted to logarithmic values and used for statistical comparison of two kits. T-test was used to study the statistical difference between two methods. The statistical comparison and the linearity between the two results were determined by Bland-Altman plot and Passing-Bablok analyses respectively. Results: Log HBV-DNA results in Qiagen and Iontek kits were within the 95% confidence interval of the bias (-0.84; standard deviation: 0.67). There was no significant difference and the relationship between two variables was linear. Conclusion: The comparative distribution analysis of Qiagen and Iontek kits indicated that a product produced in our country can be safely used in the treatment follow-up of patients with CHB. These type of studies may support the production of high value-added products in our country and also they can be utilized by other users in the world.
Authors and Affiliations
Ayse Arikan, Murat Sayan
Keywords
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- EP ID EP684574
- DOI 10.4274/vhd.galenos.2020.2019.0024
- Views 137
- Downloads 0
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