Comparison of Various Solutions for Temporary Storage of Umbilical Cord Derived Mesenchymal Stem Cells
Journal Title: Annual Research & Review in Biology - Year 2017, Vol 21, Issue 2
Abstract
Aims: To determine whether physiologic saline, phosphate buffered saline (PBS) and high glucose Dulbecco’s modified Eagle’s Medium (DMEM-HG) were suitable as temporary storage solution. Study Design: In vitro experimental analytical study. Place and Duration of Study: Stem Cell Medical Technology Integrated Service Unit, RSCM/Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia, from August through December 2016. Methodology: We did viability assessments at various time points, namely after 0, 3, 6, 24, 48, 72, 96, and 168 hours in the three storage solutions. For population doubling time (PDT) assessments the cells were re-cultured after various time points in the tested solutions. All viability and PDT assessments were done in four replications. Furthermore, for all storage solutions, differences between the various time points in terms of viability and PDT were compared and tested by ANOVA or Kruskal-Wallis test. Results: Viability was >70% up to 72 hours in PBS and physiologic saline, and up to 96 hours in DMEM-HG. Decrease in viability began to be significant compared to initial viability after 6, 24, and 72 hours in PBS, physiologic saline, and DMEM-HG respectively. Increase in PDT began to be significant compared to initial PDT after 3, 6, and 24 hours in PBS, physiologic saline, and DMEM-HG respectively. Conclusion: For our UC-MSCs, DMEM-HG is the best temporary storage solution, and storage should not exceed 24 hours.
Authors and Affiliations
Stanislaus Ivanovich Krishnanda, Raksheeth Agarwal, Oliver Emmanuel Yausep, Muthia Rizkita, Ria Angraeni, Jeanne Adiwinata Pawitan
Keywords
Related Articles
- EP ID EP313549
- DOI 10.9734/ARRB/2017/38233
- Views 101
- Downloads 0
Root Extract of Purwoceng (Pimpinella pruatjan) Enhances Aggressiveness, but Not Libido, in Male Mice
Aims: Purwoceng herbs, Pimpinella pruatjan Molk., are widely marketed in Indonesia; it is believed that the plant was used by ancient Javanese kings as an aphrodisiac. Unfortunately, the claim that purwoceng is an effect...
Callus Induction from Carob (Ceratonia siliqua L.) Seedlings and Leaves of Mature Tree
Callus induction was successfully carried out from several explants of carob (Ceratonia siliqua L.). Callogenesis from the apex was tested on three different media containing Woody Plant Medium (WPM), Murashige and Skoog...
Bivalve Superpower: The Global Invasion of Corbiculid Clams
Corbiculidae is a family of clams which has the capability of invading habitats. Particularly, there are three genera (Batissa, Polymesoda, Corbicula) which are widely distributed all over the world. The genus Batissa te...
Cognitive Function in Male Track and Field Iranian National Team Athletes
Background: Cognitive function including decision making and short term memory are affected by environmental factors including physical activity. Data showed that sustained physical activity can improve learning and memo...
Primary, Secondary Metabolites, Biochemical and Antioxidant Activity of Orthosiphon staminues Benth (Misai Kucing) Under Cadmium Exposure
Aims: This study was conducted to evaluate the effects of cadmium (0, 3,6, 9 mg/kg) on the primary, secondary metabolites, biochemical and antioxidant activity in Orthosiphon stamineus. Study Design: Orthosiphon stamine...