COMPARISON STUDY BETWEEN IN-HOUSE IGM DOT-ELISA AND THE MICROSCOPIC AGGLUTINATION TEST (MAT) FOR THE DIAGNOSIS OF HUMAN LEPTOSPIROSIS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 4
Abstract
An indirect enzyme-linked immunosorbent assay (ELISA) was compared with the microscopic agglutination test (MAT) for the diagnosis of bovine leptospirosis. Blood samples from a total number of 319 HBsAg negative suspected leptospirosis case’s were received from Government Hospital and from a few private hospitals of Salem district, Tamilnadu, India. The serum samples were examined for the presence of anti leptospiral antibodies using a commercial qualitative method of an in-house Dot-ELISA assay and the results were compared with WHO standard Microscopic Agglutination Test (MAT). The following interesting results were noted, 132 (41.7 %) serum samples were positive to Dot-ELISA, while 130 (40.7 %) were positive to MAT. All samples positive to MAT were positive to Dot-ELISA, on of the samples were positive for MAT and negative to Dot-ELISA. The Dot-ELISA showed 100% sensitivity compared to MAT. The current diagnostic Dot-ELISA appears as a rapid, non hazardous and better alternative to MAT for the diagnosis of human Leptospirosis.
Authors and Affiliations
Ganesh Kumar , V Rajasekar , G Selvakumar , DP Dhanalakshmi , S Nandagopal
Keywords
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