Complex of Small Non-coding RNAs piR-30074 and Antago-miR-155 and miR-125b with DDMC Carrier Transforms Girardi Heart Cells into CD4+ Cells
Journal Title: Journal of Cancer and Tumor International - Year 2016, Vol 4, Issue 4
Abstract
Girardi heart cells are cell culture of right atrial cells contaminated with HeLa cervical adenocarcinoma cells. The combined cells have genotypic characteristics of cervical cancer. In this study, was investigated influence of complex of DDMC vector with small non-coding RNAs (sncRNAs) on Girardi Heart cells. As the result, the genotypic and phenotypic characteristics of Girardi heart cells were changed. Micro-RNAs (miRs) and pi-RNAs (piRs) are sncRNAs, a class of small epigenetic regulatory molecules that control normal development and differentiation of different cell types. For transfection, the cells were treated with a complex of non-viral DDMC vector with piR-30074 and antago-miR-155, followed by the addition of a complex of DDMC vector with miR-125b. I observed OCT4 gene and protein expression in the cells after treatment using the complexes of DDMC vector with piR-30074 and antago-miR-155. The expression of KIR2DL1, GITR1, DICER1, PIWIL1, RISC1, FLT3, NOS2, MAFB, LIN28 genes was detected and the expression of CD4+ and CD117+ protein markers was observed. The expression of these genes and proteins was not observed in control cells.
Authors and Affiliations
Oxana V. Klimenko
Keywords
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- EP ID EP243364
- DOI 10.9734/JCTI/2016/30472
- Views 75
- Downloads 0
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