Computational Molecular Analysis of the Sequences of PAPPA2 Gene of Selected Ruminants and Non Ruminants
Journal Title: Biotechnology Journal International - Year 2017, Vol 19, Issue 1
Abstract
Pregnancy associated plasma protein A2 (PAPPA2) is an Insulin-like growth factor binding protein (IGFBP) protease of the pappalysin family. This gene has been reported to be associated with prenatal growth, postnatal growth, skeletal growth, calving interval, milk yield, fertility and parity in cattle. The present study was undertaken to computationally investigate the attendant effects of the genetic variants of the PAPPA2 gene on its function and to gain insight into the evolutionary proximity and divergence in ruminants and non-ruminants at the studied locus. A total of fourteen (14) PAPPA2 nucleotide sequences comprising cattle (3), sheep (4), goat (1), pig (3), chicken (1) and horse (2) were retrieved from the GenBank. Functional analysis of non-synonymous single nucleotide polymorphism showed that eight amino acid substitutions (C31I, R60V, Y71G, S119L, S181Y, R190I, Q361M, P178_E180del) in goats, seven in sheep (V9E, L44D, T185N, A125W, Y78S, R194V, R240L), seven in cattle (L11F, C50_W51insTDAPK, E100L, A250T, G257L, M850V), nine in chickens (A60G, S104_R170del, A190F, G128A, I10C, S309G, F48L, Q1630L) and eight in pigs (A61L, P72D, L11Q, K184T, D110C, S193_R194insTQD, Q481_E484del and T170_V172delinsVA) were returned neutral suggesting their beneficial effect. The phylogenetic trees from nucleotide sequences revealed the close relatedness of members of the Bovidae family (sheep, cattle and goat). The present information may be exploited in research into the association between PAPPA2 genotypes and some important economical traits in farm animals.
Authors and Affiliations
M. Omolara Akinyemi, H. Osamede Osaiyuwu, Ismail A. Adegoke
Keywords
Related Articles
- EP ID EP315152
- DOI 10.9734/BJI/2017/33125
- Views 128
- Downloads 0
Effects of Moringa Oleifera Linn Seed Administration on Sperm Production Rate and Gonadal Sperm Reserve in Rabbits
Aim of Study: To investigate the effects of oral administration of moringa seed on sperm production rate and gonadal sperm reserve in rabbits. Study Design: 7 Month old mixed breed male rabbits were randomly assigned int...
Molecular Identification of Aspergillus flavus Using Inter Transcribed Spacers (ITS)
Infections caused by Aspergillus have grown in importance in the last decade. Outbreaks which are caused by Aspergillus flavus appeared to be related to the single or closely related fungal strains. The fungi produce a c...
Sensitivity, Approach and Awareness about Genetically Modified Crops among Undergraduate Students: An Online Survey
The Survey-based studies provide the guidelines and feedback for policy makers about adopted and newborn technology. Biotechnology is the new branch of applied science and is emerging as the separate branch of the study...
Characterization and Antimicrobial Susceptibility Patterns of Isolates from Ward Fomites
Aim: The study was conducted to determine antimicrobial susceptibility patterns among isolates from ward fomites at Kiwoko Hospital and to detect resistances in the form of Macrolide Lincosamide StreptograminB (MLSB), Me...
Isolation and Characterization of Lactic Acid Bacteria Used for Ensiling Bamboo-shoot Shell
Aims: To construct a stable and functional lactic acid bacteria community that can be used in the ensilage of bamboo-shoot shell to improve the taste and flavor. Methodology: Using naturally fermented bamboo-shoot shell...