Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus
Journal Title: Journal of Applied Veterinary Sciences - Year 2017, Vol 2, Issue 1
Abstract
Through the present work, anti-FMD IgY immunoglobulins for serotypes O; A, and SAT2 were prepared in chicken egg yolk through immunization of laying hens with locally prepared trivalent FMD vaccine. The antibody titres against three serotypes (O; A, and SAT2) measured by SNT were 2.58; 2.8 and 2.6 respectively and were 2.81; 2.81 and 2.8 respectively when measured by ELISA. Evaluation of Anti-FMD IgY conjugated with Horseradish peroxidase for detection of FMD virus in 100 tongue epithelium; 50 OP fluid samples; 20 nasal swabs from naturally infected cattle, and 100 tissue culture infected fluids revealed sensitivity of 85% indicating the success of preparation of anti-FMD immunoglobulins conjugated with horseradish peroxidase as local product available on request saving cost and time.
Authors and Affiliations
Ehab El-Sayed Ibrahim, Hamed Adel Hamed El-Helw, Nermen Guda Shafik, Manal Awad Mekhail
Keywords
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- EP ID EP677181
- DOI 10.21608/JAVS.2017.65605
- Views 309
- Downloads 1
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