Construction of Indonesian-Strain Avian Flu Virus Seed Vaccine Using Low Pathogenic Hemagglutinin Gene and Neuraminidase Pr8 Gene through Reverse Genetics
Journal Title: Journal of Vaccines and Immunology - Year 2017, Vol 3, Issue 1
Abstract
Avian Influenza H5N1 has been spreading in Indonesia since 2003. The Avian influenza virus H5N1 actually infects various animals, including birds, mammalian and others. Since 2005, avian influenza virus has infected humans. According to data, there are 132 persons suspected of contracting this virus, and this was one of the highest cases of avian influenza H5N1 outbreak in the world. One of the means of protection against infection is vaccination. Until now there is no H5N1 influenza vaccine extant, because the virus has a high level of pathogenicity, which makes it difficult to obtain a high yield of seed vaccine. In this research, we constructed H5N1 virus with low pathogenic Hemagglutinin gene and neuraminidase gene from PR8 (H1N1) using reverse genetic method. The new virus then inoculated in embryonated chicken eggs. We tested the H5N1 virus reverse genetic using molecular methods such as PCR and sequencing; and we used EID50 and Intravenous Pathogenicity Index for the pathogenicity test. The new H5N1 virus reverse genetic was also tested in mice to observe the antibody’s titer and challenge.
Authors and Affiliations
Nidom Reviany Vibriaanita, Alamudi Muh Y, Sillehu Sahrir, Indrasari Setyarina, Suindarti Rahmalia D, Qurnianingsih Ema, Dachlan Yoes P, Syahrani Ahmad, Rachmawati Kadek, Santoso Kuncoro P
Keywords
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- EP ID EP553761
- DOI 10.17352/jvi.000021
- Views 52
- Downloads 0
Construction of Indonesian-Strain Avian Flu Virus Seed Vaccine Using Low Pathogenic Hemagglutinin Gene and Neuraminidase Pr8 Gene through Reverse Genetics
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