Content of protein fractions of blood plasma in animals under the conditions of protein deficiency
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2015, Vol 7, Issue 1
Abstract
In work considered the researches the content of protein fractions in blood plasma of rats under conditions of alimentary protein deficiency. Much attention is paid to the determination of total protein content in the blood plasma of animals. In order to study the characteristics changes of the data of biochemical parameters in the conditions of alimentary deprivation protein research animals kept for 28 days on a semi-synthetic low-protein diet based on the principle of paired food. For research use sodium citrate blood taken from the vena portae hepatis conventional method. Plasma obtained by centrifugation. Determination of the concentration of total protein in blood plasma was performed biuret method. The proportion of albumin, α-, β- and γ-globulin was determined turbidimetry method that is based on the fact that phosphate solutions of certain concentration precipitated protein fraction of blood plasma to form a suspensions, which determine the degree of turbidity nephelometric at a wavelength of 590 – 670 nm. Found that the shortage of protein in the diet of animals seen the development of hypoproteinemia due to lower total protein concentration in blood plasma. It should be noted that the decrease in the concentration of total protein in the blood plasma of experimental animals is due to reduce the content of albumin and indicates the presence of hypoalbuminemia. At the same time staying in rats low-protein diet accompanied by increased content of α- and γ-globulin plasma fractions animals. It should be noted that the level of α-globulin is increased only at the expense α1-protein fraction whose value significantly higher than those in the control group. As for α2- and β- globulin, verification of changes in their content a shortage of protein not found us. Under conditions protein deficiency rate of albumin-globulin ratio is in the range of 0.83, indicating a decrease in reserve readily available amino acids and increased catabolism of proteins under these experimental conditions, which in turn may be a primary cause development of pathological conditions of the liver.
Authors and Affiliations
Галина Копильчук, І. Бучковська, Р. Ніколаєв
Keywords
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