Correlation of Nitrite, Leucocyte Esterase and Protein Detection to Diagnosis of Urinary Tract Infections

Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 13, Issue 9

Abstract

Aim: To determine the relationship between detection of nitrite, Leucocyte esterase (LE) and protein in urine and significant bacteriuria. Study Design: Cross-sectional descriptive study. Place and Duration of Study: Department of Medical Microbiology and Parasitology, University of Port Harcourt Teaching Hospital, between March and September 2015. Methodology: 240 urine samples were analyzed. Dipstick analysis using Combi-UriScreen 10SL reagent strips (Axiom Medical limited, UK) and culture for significant bacteriuria were performed according to manufacturer’s instruction/ using standard protocols. Data was coded, entered into Microsoft Excel ® version 2010 and analysed using Epi-Info version 7.02. Categorical data were presented as frequencies and percentages using tables. Univariate analysis using logistic regression (Odds Ratio) was used to determine the association between the presence of nitrite, LE and protein and significant bacterial yield in urine. A P-value of ≤ 0.05 was considered statistically significant. Likelihood ratios were calculated. Results: 23 (23.2%) out of 99 samples with significant bacteriuria were nitrite positive, while 42 (42.4%) and 45 (45.5%) were positive for leucocyte esterase and protein respectively. Nitrite (P = 0.001, OR = 5.03, 95% CI = 2.02-12.93) and leucocyte esterase positivity (P = 0.001, OR = 3.59, 95% CI = 1.91-6.80) were significantly associated with significant bacteriuria while proteinuria was not (P = 0.989, OR = 1.03, 95% CI = 0.60-1.79). Nitrite positivity alone had the best positive likelihood ratio (4.09, 95% CI: 1.91, 8.78) followed by the combination of nitrite and LE positivity (3.65, 95% CI: 1.90, 7.03). Conclusion: The use of dipstick analysis of urine as a screening tool for samples to be cultured may be a very effective way of reducing laboratory costs and wastage of man hours, which both ultimately improve the effectiveness of clinical laboratories especially in resource-poor settings.

Authors and Affiliations

K. T. Wariso, I. L. Oboro

Keywords

Related Articles

Birth Weight and Placental Weight Correlation of Fulani Ethnic Group Parturients in Nigeria

Aims: We aim to establish the relationship of birth weight to the placental weight of Fulani ethnic community which is an important population politically and economically not only in Nigeria but to West Africa in genera...

Oxidative Stress Pathway Mechanisms Induced by Four Individual Heavy Metals (As, Hg, Cd and Pb) and Their Quaternary on MCF-7 Breast Cancer Cells

Cell death induced by the production of reactive oxygen species (ROS) has largely been associated with the activation of oxidative stress pathway; however, the direct mechanism(s) involved are unknown. This study evaluat...

Insulinomimetic, Antihyperlipidemic and Antioxidative Properties of Azadirachta indica. Possible Mechanism of Action

Currently available synthetic drugs for management of diabetes mellitus have been reported to cause side effects, hence searches for safer, readily available and cheaper sources among medicinal plants. One hundred and th...

A Rare Highly Aggressive Tumor of the Kidney: The Pure Epithelioid Angiomyolipoma

Aims: The authors presents a case of pure Renal epithelioid angiomyolipoma (EAML) and discuss the clinicopathological characteristics and the diagnosis problems of this entity. Case Presentation: A 51 year-old man pre...

Impact of Prosthetic Characteristics and Life Stress on Oral Health Related Quality of Life in Anterior Fixed Dental Prosthesis Treatment

Aims: This cross-sectional study aimed to explore potential factors influencing oral health related quality of life (OHRQoL) in a Chinese population treated with anterior dentition fixed dental prosthesis (FDP). Study De...

Download PDF file
  • EP ID EP340848
  • DOI 10.9734/BJMMR/2016/23444
  • Views 53
  • Downloads 0

How To Cite

K. T. Wariso, I. L. Oboro (2016). Correlation of Nitrite, Leucocyte Esterase and Protein Detection to Diagnosis of Urinary Tract Infections. Journal of Advances in Medicine and Medical Research, 13(9), 1-6. https://europub.co.uk./articles/-A-340848