Correlation of VITEK -2 and conventional tests in identification of Non Fermenting Gram Negative Bacilli in a Tertiary Care Hospital
Journal Title: Indian Journal of Microbiology Research - Year 2018, Vol 5, Issue 2
Abstract
Introduction: The non-fermentative Gram negative bacilli (NFGNB) are a group of aerobic, non-spore forming, Gram negative bacilli that either do not utilize carbohydrates as a source of energy or degrade them through metabolic pathways other than the fermentation. Material and Methods:The study was undertaken to identify various non- fermenters isolated from patients in our hospital, a Tertiary Care Center at Uttarakhand. 252 (14.15%) non-fermenters were isolated from 1780 clinical specimens on MacConkey and blood agar plates incubated at 35°C for 24-48 hours. Any growth that was identified as non-fermenters by Vitek-2 was subjected to identification by additional conventional tests. Results and Conclusion: Patients mostly belonged to age group of 51 to 70 years and were commonly male. Sputum & ET secretion were the commonest samples. Psuedomonas aeruginosa was the commonest isolate followed by Acinetobacter calcoacetecus baumanii complex. Out of total 252 isolates, 221(87.69%) could be accurately identified upto species level by conventional tests. The conventional tests could identify 243 (96.42%) isolates upto genus level. Majority of them showed multidrug resistance. The most effective antibiotics were Tigecycline and Polymyxin B/ Colistin.
Authors and Affiliations
Shweta Sharma, R K Agarwal, Barnali Kakati
Keywords
Related Articles
- EP ID EP472909
- DOI 10.18231/2394-5478.2018.0042
- Views 75
- Downloads 0
Evaluation of antimicrobial resistance in urinary isolates of member of enterobacteriaceae among woman attending Tertiary Care Hospital
Introduction: 1. Urinary Tract Infections (UTIs) are one of the most common urogenital infections and affects all age groups including men, women and children worldwide. 2. Urinary tract infections are a major public hea...
Comparison of Phenotypic and molecular profile of Coagulase negative Staphylococci from clinical isolates and commensals along with Biofilm detection
Background: Coagulase-Negative Staphylococci (CoNS) are commensals of the human skin and mucosa. However, Staphylococcus epidermidis (S. epidermidis) and other CoNS have emerged as nosocomial pathogens. This study aims t...
Different Methods of Staining to optimize Smear Microscopy for Diagnosis of Pulmonary and Extra Pulmonary Tuberculosis
Introduction: Tuberculosis continues to be a major public health problem especially in low income and high incidence countries like India. Aim of the study was to access and compare sensitivity and specificity of Light...
Bacteriological profile of post surgical wound infections: A one year retrospective study from a government orthopaedic hospital
Post-operative wound infection is a severe problem in the surgical specialization and is an important cause of morbidity and mortality. In most of these wound infections the causative agents arise from the endogenous flo...
Evaluation of Transient Microbial Flora on Biometric System as a Potential Source of Infection in Tertiary Care Hospitals
In developing countries, along with the Health Care Providers, Inanimate objects plays a critical role in microbial transmission so also the ability of the microorganism to survive on inanimate objects to cause Hospital...