Creation of gene organisms in starting and ending regions of SicA gene of Salmonella enteritidis in pGEM vector
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 6, Issue 1
Abstract
Salmonella enteritidis is the most common cause of gastroenteritis. Sic A and Inv F activates transcription of sopB / sigD and sopE which coding effectively secreted proteins that enhance the code to be offensive. In this study upstream and downstream regions SicA gene of Salmonella enteritidis in pGEM vector was performed. In this study, the upstream and downstream genes SicA Salmonella enteritidis amplified based on specific primers and PCR and The fragment DNA cloned by T/A coloning method in pGEM vector and this constarct transformed into E. coli. The upstream and downstream SicA genes of Salmonella enteritidis was confirmed using PCR. The result of next steps showed that these regions were successfully cloned in E. coli. Confirmation of this conestract was done by digestion of restriction enzyme. According to the results ,can produce the conectruction via the recombination homologous and insertion of anti- biotic resistant genes between the upstream and downstream regions of SicA gene of Salmonella eneritidis as a candidate for gene vaccina against Salmonella eneritidis in future studies. Present study indicates the successful cloning of starting and ending encoder regions of SicA gene of Salmonella enteritidis in E. Coli bacteria. Thus, it seems that the structure produced in this study can be used as a gene vaccine candidate against Salmonella in future studies.
Authors and Affiliations
Sara Ostovari, Mohammadreza Gholami, Mohammad Zolfaghari, Ghasem Rahimi, Morteza khani
Keywords
Related Articles
- EP ID EP38185
- DOI -
- Views 130
- Downloads 0
Sowing date evaluation on phenological traits of canola genotypes under warm and semi arid climate (ahvaz region)
Evaluating the phenological traits of rapeseed crop provides the ability to manage planting based on optimal compatibility to the environmental condition. Thus, experiment on the basis of split plot arrangement in rand...
Studying the morphological traits of winter barley varieties and lines in Ardabil using multivariate statistical methods
We conducted an experiment at Agricultural Research and Natural Resources station located in east 10 km from Ardabil (Aralloo region) in 2012-2013 to study the morphological traits of few winter barley varieties and lin...
Single drop scanometry, Determination of cyanide in water in the various color spaces.
In this study, the application of new and simple single drop scanometric method was described for determination of cyanide as alternative for visible spectrophotometric method. Cyanide ion reacts with the methyl violet a...
The effect of land use changes on groundwater level decline (Case study: North of Urmia lake basin)
One of the direct impacts of land use on the hydrological situation of each region is relationship between land use changes with fluctuations in ground water table. The aim of this study was to investigate the relationsh...
Investigate of bio-mechanical method on carbon sequestration and macro-element concentration in soil and plant
Biological management and mechanical practice are available for controlling soil erosion, therefore, soil management practice, which accretion of organic carbon, nitrogen and phosphorus and it can help in improving soil...