CTX-M-15 Type β-lactamases From Clinical Isolates of Escherichia coli by Polymerase Chain Reaction and DNA Sequencing
Journal Title: Zahedan Journal of Research in Medical Sciences - Year 2017, Vol 19, Issue 3
Abstract
Background: E. coli is a common cause of urinary tract infection and bacteremia in humans. That is frequently resistant to β-lactam antibiotics. Objectives: The aim of this study was to determine the antibiotic resistance and CTX-M extended-spectrum β-lactamases (ESBL) carrying E. coli isolated in hospitals from two different areas in Iran. Methods: In this descriptive cross-sectional study, 93 clinical isolates of E. coli were collected from urine culture of inpatients in Mashhad and Qom hospitals from August 2013 to February 2014. Bacteria were re-confirmed by Microgen kit. Antimicrobial and ESBL tests were performed using by standard disc diffusion method. Polymerase chain reaction (PCR) was used for detection of plasmid mediated CTX-M-1 and CTX-M-2 ESBLs. Four of the blaCTX-Mgenes were sequenced. Statistical analysis carried out using SPSS (version 16) software and Chi-square test. Results: High prevalent isolates were resistant to ampicillin, cephalothin, nalidixic acid and cotrimoxazole, but sensitive to imipenem, nitrofurantoin and amikacin. The ESBL phenotype was detected in 48 (51.6%) isolates, 42 of which were carrying blaCTX-Mgene. There was no significant difference between the β-lactamase-producing bacteria from the two studied cities. 81% and 85% of blaCTX-Mcarriers from hospitals in Qom and Mashhad were respectively found to have CTX-M-1 ESBL, whereas none of them showed CTX-M-2 ESBL cluster. BlaCTX-M-15was found in four selected bacteria by sequencing. Conclusions: This study showed a high rate of CTX-M-1 cluster and also presence of CTX-M-15 ESBL in two different areas of Iran, which emphasize the need for employing an excellent management program in antibiotic therapy.
Authors and Affiliations
Mahboobeh Nakhaei Moghaddam, Mohammad Reza Zolfaghari, Niloofar Tavakoli‐Hoseini
Keywords
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