CYTB: A HOT SPOT FOR PATHOGENIC MUTATIONS IN MITOCHONDRIAL GENOME OF BREAST CANCER AND OVARIAN CANCER PATIENTS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 9
Abstract
Objective: Out of various cancer types, Breast and ovarian cancers are the most commonly occurring malignancies in women. As per literature, a large number of mutations are reported in various mitochondrial genome encoded subunits of respiratory chain complexes in breast and ovarian cancer patients. However, a very few of them are functionally validated till now. Our study is an attempt to highlight the pathogenic potential of all these reported mutations in breast and ovarian cancer patients.Methods: In order to achieve so, total 109mitochondrial gene mutations of breast cancer and 11 mitochondrial gene mutations of ovarian cancer patients were selected from MITOMAP database as well as various literatures. All these mutations were analyzed using various in silico tools such as MUSCLE, PolyPhen-2, SIFT, Mut Pred, Mu Pro, PANTHER, GOR4 and MUSCLE.Results: As a result of our analysis, 28 out 95 mutations in CytB gene are most pathogenic in the case of breast cancer patients. On the other hand 2 out of 3 mutations of the same gene were predicted to be potentially pathogenic in case of ovarian cancer patients. Mutations in other mitochondrial subunit was also predicted pathogenic but with the low score.Conclusion: Out of different mitochondrial subunits, CytB seems to most important site for mutations in these two groups of patients. Hence, mutations of CytB subunit, which are predicted to be highly pathogenic as per our analysis, should be functionally validated in future.Â
Authors and Affiliations
Nancy Taneja, Samiksha Kukal, Shalini Mani
EVALUATION OF ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF FENUGREEK (TRIGONELLA FOENUM-GRAECUM) EXTRACTS
Objective: The present investigation was performed to evaluate the antimicrobial potential of Trigonella foenum-graecum leaves extracts and to elucidate the presence of phytochemicals responsible for its biological activ...
DRUG USE INDICATORS IN PATIENTS WITH TYPE 2 DIABETES IN A TERIARY HEALTHCARE FACILITY IN NIGERIA
Objective: The study analyzed the utilization pattern of antidiabetic drugs at the outpatient clinic of a teaching hospital in Nigeria to document information for enhancing the rational use of drugs in type 2 diabetes.Me...
THE PROTECTIVE ROLE OF FLAXSEED LIGNAN IN MALE RABBITS WITH HIGH-FAT DIET: HISTOPATHOLOGICAL STUDY
Objective: The objective of the study was to investigate the effect of dietary flaxseed-derived lignan supplement as anti-hyperlipidemic (fatty liver changes) on rabbit liver.Methods: Rabbits were assigned randomly into...
EFFECT OF SELECTED POLYMERS ON DISSOLUTION AND STABILIZATION OF AMORPHOUS FORM OF MELOXICAM
Objective: Meloxicam is classified as class II corresponding to its high permeability and low solubility (12μg/ml). This study aims to compare the effect of selected polymers on stabilization of amorphous form, and diss...
FORMULATION AND CHARACTERIZATION OF ASCORBYL PALMITATE LOADED O/W MICROEMULSION
Objective: Ascorbyl palmitate (AP) is an effective free radical-scavenging antioxidant which promotes skin health and vitality. Besides that, AP helps to enhance the synthesis of collagen. Moreover, AP helps to reduced U...