Cytokines TNF-Alpha and IL-8 Gene Polymorphisms in Sickle Cell Anaemia Patients under Hydroxyurea Treatment
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 6
Abstract
ABSTRACT Introduction: Sickle Cell Anaemia (SCA) is an inherited disorder characterised by homozygosis for the mutation that causes Haemoglobin S (Hb S) production. SCA patients have increased serum levels of circulating Tumour Necrosis Factor Alpha (TNF-α) and Interleukin 8 (IL-8) during crisis events, occasions because of increased production from inflammatory cells, glia and neurons, these inflammatory molecules additionally contribute to the advanced mechanisms concerned in vascular occlusion events. Aim: This study aimed to investigate polymorphisms in the TNF-alpha and IL-8 genes, their association with the haematological changes, to investigate the association between the TNF-alpha and IL-8 gene polymorphisms and the TNF-alpha and IL-8 serum levels in Saudi SCA patients presented with or without treatment in comparison to healthy individuals. Materials and Methods: The study included 87 SCA patients diagnosed as homozygous for Haemoglobin S (Hb S; using haemoglobin electrophoresis methods and High-Performance Liquid Chromatography (HPLC) and attended at hereditary blood disease centre (Al-Ahsa; Saudi Arabia) for follow-up. The patients of both genders in all age groups were subdivided into two groups; 27 of the patients were undergoing hydroxyurea treatment (AHU) and 60 patients without hydroxyurea treatment against 30 healthy individual setting as control group. The collected data were analysed using the STATA SE 10 and GraphPad Prism 5.0. Results: TNF-α and IL-8 levels were significantly higher within the plasma of SCA individuals compared to control individuals. The GG and AA genotypes of TNF-alpha-308G>A were associated with the increase in the serum levels of TNF-alpha in SCA patients. While, AA and TT genotypes -251A>T IL-8 gene polymorphism was associated with increase in the serum levels of IL-8 in SCA patients. Conclusion: The haematological investigations of SCA further highlight the contribution of genetic modifications to the risk of clinical genotypes to understand the association of serum levels of TNF-α and IL-8 in patients under HU compared to their gene polymorphism.
Authors and Affiliations
Fathelrahman M Hassan, Faisal M Alzahrani
Keywords
Related Articles
- EP ID EP523102
- DOI 10.7860/JCDR/2018/35740.11681
- Views 72
- Downloads 0
Evaluation of Gengigel® Application in the Management of Furcation with Coronally Advanced Flap through Surgical Re-Entry- A Split Mouth Clinical Study
Introduction: One of the challenging and unique periodontal problem of Grade II furcation defect has been managed through different treatment modalities in the past. A successful approach is based on complete closure of...
Wheatgrass: Green Blood can Help to Fight Cancer
Introduction: Oral squamous cell carcinoma is the sixth most common cancer worldwide and is one of the leading causes of death especially in developing countries like India. It is usually managed with the help of chemoth...
Combination of Proximal Fibulectomy with Arthroscopic Partial Meniscectomy for Medial Compartment Osteoarthritis Accompanied by Medial Meniscal Tear
ABSTRACT Introduction: Medial compartment osteoarthritis is usually accompanied by medial meniscal tear. The treatment of symptomatic mild medial compartment osteoarthritis and medial meniscal tears in middle-aged and el...
A Comparative Study of Analgesic Effect of Intrathecal Nalbuphine and Fentanyl as Adjuvant in Lower Limb Orthopaedic Surgery
Introduction: Intrathecal opioids when added to local anaesthetics decrease their dosage and provide haemodynamic stability. Nalbuphine is an agonist-antagonist and acts on kappa receptors providing analgesia. Aim: The s...
The Effect of Metoclopramide Addition to Lidocaine on Pain of Patients with Grades II and III Post-Episiotomy Repair
Introduction: Episiotomy is the most common procedure used for dilatation of the vaginal opening for giving birth. Although episiotomy is associated with benefits for the mother, it may lead to short term and long term d...