Cytological Assessment of Barr Bodies in Buccal Mucosal Smears and Peripheral Smear in Sex Determination
Journal Title: Indian Journal of Forensic Medicine and Pathology - Year 2017, Vol 10, Issue 3
Abstract
Introduction: Barr body has been recognized as the cytological manifestation of the inactive X chromosome in interphase nuclei in a highly condensed state. Aims and Objectives: To assess the efficacy of sex determination between pap stained buccal mucosal scrapes and leishman stained drumstick appearance in peripheral smear. Material and Methods: Buccal mucosal smears and peripheral smears are collected from 100 individuals who visit K. R. hospital and Cheluvamba hospital of age ranging from 20-30 years. The buccal mucosal scrapes were stained with papanicolaou stain and peripheral smear of the same individual were stained with leishman stain. Results: In females and males drumstick appearance was more compared to barr body. Conclusion: Sex determination using buccal scrapes and peripheral smear stained with papanicolaou stain and leishman stain proved to be simple, accurate and cost effective method in our study.
Authors and Affiliations
Karra Shilpa
Keywords
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- EP ID EP456036
- DOI 10.21088/ijfmp.0974.3383.10317.4
- Views 73
- Downloads 0
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