Cytotoxic and Apoptogenic Properties of 2-Phenylthiazole-4-carboxamide Derivatives in Human Carcinoma Cell Lines
Journal Title: Journal of Reports in Pharmaceutical Sciences - Year 2012, Vol 1, Issue 1
Abstract
Apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development and tissue homeostasis. Caspases, a family of cysteine-dependent aspartate-directed proteases, play a critical role in the initiation and execution of apoptosis. In this study, cytotoxicity and apoptogenic effect of 2-phenyl 4-carboxamide derivatives were evaluated in SKNMC (human neuroblastoma), MCF-7 (human breast adenocarcinoma) and HT-29 (human colon cancer) cell lines. Cell viability was determined by MTT assay. Also, activation of caspase-3 was evaluated by spectrophotometry. The overall cytotoxicity profiles of derivativesA demonstrated that the HT-29 cell line has more sensitivity respect to other cell lines. Moreover, our observations indicated that 3-F and 2-F derivatives and A 4-Cl derivative increased caspase-3 activation in three carcinoma cell line compared to control.A Collectively, these findings suggest that these derivatives areA able to induce apoptosis in cancer cell lines.
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