Cytotoxicity of Various Fractions of Compounds Extracted from Yoyo Bitters on Human Cervical Cancer Cells
Journal Title: European Journal of Medicinal Plants - Year 2015, Vol 7, Issue 2
Abstract
Aims: To investigate cytotoxicity of pure fractions of compounds extracted from yoyo bitters on human cervical cancer cell and to characterize the compounds present in various extracts. Study Design: Experimental Study. Background: Cervical cancer is one of the leading causes of death. It is common among women of reproductive age and it causes the highest morbidity and mortality among this population. In spite of several researches, therapeutic cure for cancer using conventional therapies has been elusive and this has resulted in the use of alternative therapies. Yoyo Bitters (YYB), a herbal product, produced in Nigeria is widely consumed with a view to alleviating various health challenges. Previous studies using animal models have tried to elucidate the pharmacological activities of YYB, but none has explored its cytotoxicity against human cervical cancer cells. This study was designed to investigate the cytotoxicity of pure fractions of compounds extracted from yoyo bitters on human cervical cancer cell and to characterize the compounds present in various extracts. Materials and Methods: Pure extracts of YYB were obtained using Column Chromatography. These extracts were investigated in vitro on Human Cervical Cancer Cells (Hela) with low passage number using MTT assay. Chemical compounds present in the extracts were characterized using Gas Chromatography, Mass Spectrometry (GC-MS). Results: showed that various fractions of YYB exhibited cytotoxic activities on human cervical cancer cells. Half maximal (IC50) of fractions 1, 2 and 3 on cervical cancer cells were: 35.3 µg/ml, 5.6 µg/ml and 44.4 µg/ml respectively. The IC50 of Fraction 2, 5.6 µg/ml, was statistically significant (P= 0.008) when compared with values obtained from fractions 2 and 3. Conclusion: This study showed that all fractions of YYB exerted cytotoxic activity, but chemical compounds contained in fraction 2 exerted the highest cytotoxicity. Pharmacotherapeutic application of the chemicals as antitumorigenic agent against cervical cancer is discussed.
Authors and Affiliations
A. A. Onyeaghala, I. O. Omotosho, A. R. Shivashankara
Keywords
Related Articles
- EP ID EP350134
- DOI 10.9734/EJMP/2015/15913
- Views 110
- Downloads 0
Evaluation of Root Extract of Acacia nilotica on Haematological and Lipid Profile in Rats
Aim: Acacia nilotica root has been used for different medicinal purposes wherever the plant is found in Nigeria. This study was designed to evaluate the effects of Acacia nilotica aqueous root extract on hematological pa...
Lemon Balm (Melissa officinalis L.) Water Requirement, Crop Coefficients Determination and SIMDualKc Model Implementing
The crop water requirements and coefficients should be determined for proper irrigation management and scheduling. The present study was conducted to determine water requirements, and single and dual crop coefficients of...
Antischistosomal Activity of Azadirachta indica and Ekebergia capensis in Mice Infected with Schistosoma mansoni
Aims: Schistosomiasis is a parasitic disease of great socio-economic and public health importance in tropical and sub-tropical countries. Praziquantel (PZQ) is the drug of choice for treatment of schistosomiasis since it...
The Hypoglycemia Effect of Alkaloid Compounds from Oil Free Mahagony Seeds (Swietenia macrophylla, King)
Aim: To study the hypoglycemia effect of alkaloid compounds (Alkaloid crude extract) from oil free mahagony seeds (Swietenia macrophylla, King). Study Design: Experiments of alkaloid compounds isolation and in vivo test...
Amelioration of Chronic Unpredictable Mild Stress-Induced Behavioural Perturbations by Noni Juice in Mice: Possible Involvement of Antioxidant System
Aims: Noni juice is a natural herbal formulation containing Morinda citrifolia widely acclaimed as an immune system stimulant and mood enhancer. The aim of the study was to explore the antidepressant- and anxiolytic-like...