Dagaa (Rastrinoebola argentea) protein hydrolysate as a nitrogen source in microbial culture media
Journal Title: Journal of Applied Biology & Biotechnology - Year 2017, Vol 5, Issue 3
Abstract
Dagaa is a small pelagic fish found in Lake Victoria. It is used as a nutrient source in animal feeds and human consumption. Utilization of fish protein hydrolysate as a nutrient in culture media has been explored in several fish species and dagaa protein hydrolysate (DPH) can also be a candidate due to its rich protein and lipid content. Potential of DPH as a nitrogenous source in microbial growth media was assayed using the following bacteria; Escherichia coli, Pseudomonas aeroginosa, Bacillus subtilis and Rhodobacter capsulatus and two fungi, Metarhizium anisopliae and Beauveria bassiana. Cell growth was evaluated using turbidity and biomass in liquid cultures. DPH formulated media produced similar cell growth when compared to positive controls made up of Luria Bertani (LB) for bacteria and Sabouraud’s dextrose broth (SDB) for fungi. This indicates that formulated DPH media can be considered as an alternative media for microbial culture.
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The study aimed at optimizing the mycelial biomass production of Ganoderma lucidum by submerged fermentation. Plackett–Burman design was used to screen the important growth conditions coupled with central composite desig...
Inducible Antimicrobial Compounds (Halal) Production in Honey Bee Larvae (Apis mellifera) from Rumaida, Taif by injecting of various dead Microorganisms extracts
In the current study, the antimicrobial compounds in induced Honey bee larvae (Apis mellifera) from the Taif region were extracted from hemolymph and assigned as Halal safe product. Their minimum inhibitory concentration...
Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)
In vitro plant regeneration is preliminary requisite in the process of obtaining transgenic plants. Somatic embryogenesis is the most frequently used method of in vitro plant regeneration in maize. In this study, a relia...
Non-coding DNA – a brief review
In addition to the coding segments, genomes of all organisms are made of several highly conserved non–protein coding regions. Biochemical analysis by isolating non-coding regions from cells, tissues or whole organism stu...