Decontamination of toothbrushes used by children with special needs: microbiological analysis
Journal Title: RSBO - Year 2011, Vol 8, Issue 2
Abstract
Introduction: Children with special needs generally present poor oral hygiene, consequently, their toothbrushes become highly infected,acting as a reservoir for dissemination of different pathogens. Objective: To evaluate the contamination and decontamination of toothbrushes used by children with special needs. Material and methods: Toothbrushes were retrieved from 30 children after 30 days of use, stored in tubes containing nutrient agar and incubated for 24 h (37ºC). After, 100 µl of the dilutions (1:10 and 1:100) were plated on MacConkey agar (Gram negative bacteria), Mitis Salivarius agar (Streptococcus spp.), and Saboroud agar (Candida spp.) and incubated (37ºC, 48 h). The toothbrushes were randomly divided into 3 groups for decontamination procedures using 0.12% chlorhexidine (GC); 1% sodium hypochlorite (GH) and distilled water (GA - control). The solutions were sprayed 6 times onto the bristles and the toothbrushes were submitted to microbiological analysis. Statistical analysis used non-parametric tests (Fisher’s exact and Wilcoxon) (α = 0.05). Results: The initial microbiological analysis showed a bacterial growth in all samples. Microorganisms levels higher than ≥ 300 x 103 CFU/mL were detected in 80% of the samples for Streptococcus spp.; 60% for Gram negative bacteria e 47% for Candida spp. After decontamination, there was significant reduction for GC when considering turbidity (p = 0.04) and at the levels of Gram negative bacteria (p = 0.04) and Candida spp. (p = 0.005). For GH e GA, the decontamination procedures were not significant. Conclusion: Chlorhexidine solution was the only agent effective against Gram negative bacteria and Candida spp. None of the solutions tested was effective against Streptococcus spp.
Authors and Affiliations
Ana Cláudia Chibinski, Kamila Grando, Patrícia Fanchin, Eduardo Campagnoli, Fábio dos Santos, Denise Wambier
Keywords
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