Dendritic epidermal T cells: Their role in the early phase of ectromelia virus infection*
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2009, Vol 63, Issue
Abstract
Introduction: Dendritic epidermal T cells (DETCs) are bone marrow-derived T lymphocytes that express a canonical gd TCR and form a dense network in the murine skin. Here, we sought to determine their role during the early phase of ectromelia virus (ECTV) infection. Materials/Methods: In vivo and in vitro models were established for this purpose. In the fi rst model, C57BL/6 micewere intradermally infected into the central part of the ear pinnas with 105 PFU ECTV-Mos strain per ear. At indicated time-points, the total pinna cell population was isolated to determine the presence of DETCs and the enumeration of DETCs secreting IFN-g under in vitro stimulation. Purifi ed DETCs were also analyzed for certain gene expressions by RT-PCR. In the second model, purifi ed DETCs isolated from pinnas of uninfected C57BL/6 mice were stimulated in vitro with 5 MOI of UV-inactivated ECTV-Mos. Total RNA was isolated at indicated time-points for RT-PCR gene expression evaluation. Results: A rapid increase in DETCs number in the pinnas was observed for 24 hours post-infection. During the next 24 h the DETCs number decreased, reaching control values. Rapid but short-lasting INF-g secretion by purifi ed DETCs in vitro was observed and correlated well with the expression of the b chemokine CCL5 gene responsible for macrophage and neutrophil attraction. It was also accompanied by DETCs expression of the immunoregulatory factors TGF-b, GM-CSF, and KGF genes important for maintaining skin integrity. Conclusions: DETCs from mice infected with ECTV-Mos were rapidly induced to cascade the secretion of mediators that contribute to both immune protection and the control of skin integrity.
Authors and Affiliations
Małgorzata Gieryńska, Ewelina Pawlak, Ada Schollenberger, Irma Cespedes
Keywords
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