DESIGN AND DEVELOPMENT OF NOVEL MICROEMULSION BASED TOPICAL FORMULATION OF HESPERIDIN
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 12
Abstract
Objective: Bioflavonoid hesperidin is used primarily to assist treatment of capillary disorders like hemorrhoids and varicose veins, by reducing capillary permeability. There are certain limitations to the use of these bioflavonoid in the pharmaceutical formulations because of their physical properties like limited aqueous solubility, poor bioavailability and high oral dose. Therefore in the present research work micro emulsions of hesperidin were developed for improving solubility and bioavailability using topical route.Methods: Micro emulsions of hesperidine were prepared by plotting pseudo ternary phase diagram using eucalyptus oil, Tween 80, AccononCC6 and water. The micro emulsion with optimized droplet size, polydispersity index and ex vivo diffusion ability was then converted into the ointment for ease of application. Droplet size and Polydispersity index were obtained by using Photon correlation spectroscopy. Optimized o/w microemulsion of hesperidin composed of eucalyptus oil 20%, Tween-80/Acconon CC-6(2:1) 33% and water was then converted into ointment using optimized ointment base. [a1] The microemulsion loaded ointment was then characterized for physicochemical parameters. Ex-vivo permeation and In-vivo bioavailability studies were carried out to check the r. release profile of drug from the prepared formulations.Results: It was observed from ex-vivo permeation studies that flux value for optimized microemulsion was found to be 8.971µg/ml/cm2as compared to pure hesperidin (1.230 µg/ml/cm2). This formulation was then converted into ointment by using optimized base containing PEG-6000, PEG-400 and Cetyl alcohol. This microemulsion based ointment passed all the characterization tests (droplet size analysis using PCS, ex-vivo permeation studies, in-vivo bioavailability studies etc.) as well as remained stable for the period of 3 mo during stability studies as per ICH guidelines. The bioavailability studies of hesperidin microemulsion based ointment in rats showed 3 fold statistically significant (p<0.001) improvement in bioavailability as compared to microemulsion when applied topically.Conclusion: Thus it can be concluded that components of microemulsion and ointment are contributing to improve bioavailability of hesperidin.Â
Authors and Affiliations
Vaishali Kilor, Nidhi Sapkal, Gunjan Vaidya
Keywords
Related Articles
- EP ID EP578047
- DOI -
- Views 62
- Downloads 0
STATISTICAL OPTIMIZATION AND CHARACTERIZATION OF MICROBALLOONS FOR INTESTINAL DELIVERY OF ACID LABILE DRUG UTILIZING ACRYLIC POLYMER
Objective: The present work was to formulate buoyant system in order to protect the drug from gastric acid degradation, increasing the gastric residence time, control the drug leaching by use of enteric polymers, prolong...
PHYTOCHEMICAL ANALYSIS OF N-HEXANE LEAF EXTRACT OF ALPINIA PURPURATA (VIEILL.) K. SCHUM USING UV-VIS, FTIR AND GC-MS
Objective: The present study was carried out to characterize bioactive constituents present in n-hexane leaf extract of Alpinia purpurata (Vieill.) K. Schum.Methods: Phytochemical screening of the leaf extract of Alpinia...
ASSAY OF FOUR PSYCHOTROPIC DRUGS CHLORPROMAZINE, CLOMIPRAMINE, AMITRIPTYLINE AND NORTRIPTYLINE IN TABLETS BY A SINGLE HPLC METHOD
Objective: The objective of the study was to develop and validate a novel and single HPLC method, which is a additional practical choice in quality control laboratories, in order to assay four psychotropic drugs chlorpro...
AJUGA IVA TREATMENT INCREASES POST-HEPARIN LIPASE ACTIVITY AND DECREASES SERUM AND VLDL-TRIACYLGLYCEROLS IN RATS FED A CHOLESTEROL-RICH DIET
Objective: Hypercholesterolemia is among the most common health problems treated with traditional remedies. The role of lipoprotein lipase in hypercholesterolemia has been the subject of many reviews. We hypothesized tha...
MOLECULAR DYNAMICS STUDY OF NON-HYDROGEN-BONDING BASE-PAIR DNA DUPLEX d(GTCDNAM GCGCCGTGGC). d(GCCACGGCGCD5SICSGAC)
Objective: The objective of the study was to elucidate the structural activity of a natural DNA sequence modified by a hydrophobic base-pair which didn’t form Watson-Crick (W-C) hydrogen-bond. The modified unnatural ba...