Detection of Antibiotic Resistance Genes among Pseudomonas aeruginosa Strains Isolated from Burn Patients in Iran
Journal Title: Microbiology Research Journal International - Year 2016, Vol 12, Issue 4
Abstract
Aim: In this study, we evaluated the presence of antibiotic resistance genes among P. aeruginosa strains. Methodology: From January to September 2012, 100 isolates of P. aeruginosa were collected from burn patients. Antimicrobial susceptibility testing was performed by disk diffusion method. Screening for Metallo-β-lactamases (MBLs) productions were performed by Combination Disk Diffusion Test (CDDT). The frequency of antibiotic resistance encoding genes such as MBLs (IMP, VIM, NDM), ESBLs (CTX-M-15), Amp-C enzyme (CMY), Ambler class A carbapenemases (KPC), Ambler class D β-lactamase (OXA-48), 16S rRNA methylases (armA, rmtB, rmtC, rmtD), Quinolone Resistance Gene (aac(6′)-Ib) and class 1 integron were performed by PCR and Sequencing techniques. Results: 48(62.33%) of isolates were metallo-beta-lactamase producers. All MBL-producing P. aeruginosa were resistant to antibiotics; while 49% of isolates were resistant to Gentamicin. The aac(6)-Ib, CTX-M-15, int I, CMY, rmtB , rmtD and IMP-1 genes were detected in 57 (74.02%), 48 (62.3%), 48 (62.3%), 7 (9.09%), 11 (14.28%), 9 (11.68%) and 6 (7.7%) isolates respectively, whereas none of them were positive for other genes. The mortality rate due to metallo-β-lactamases-producing P. aeruginosa infection was 5(10.4%). Conclusions: The prevalence of antibiotic resistance genes producing P. aeruginosa detected in this study is of great concern.
Authors and Affiliations
Ali Hashemi, Fatemeh Fallah, Soroor Erfanimanesh, Alireza Salimi Chirani, Masoud Dadashi
Keywords
Related Articles
- EP ID EP350693
- DOI 10.9734/BMRJ/2016/23268
- Views 96
- Downloads 0
Changes in Serum Levels of TNF-α & IL-4 among New, Under-treatment & MDR TB Patients
Introduction: Tuberculosis (TB) remains a significant public health problem with an estimated one-third of the world’s population being infected. Cytokines play a major role in protection against Mycobacterium tuberculos...
Incidence of Antibiotic Resistant E. coli Isolated from Drinking Water Sources in Ondo, Southwestern Nigeria
Aim: The aim of this study was to determine the incidence and antibiotic resistant Escherichia coli in drinking water sources in Ondo, southwestern Nigeria. Place and Duration of Study: Department of Biological Sciences...
Analysis of the Shelf Life of Soya Bean (Glycine max) Flour
Aim: This work was to investigate the shelf life of fresh and stored soybean flour by isolating resident bacteria and carrying out their proximate analyses. Place and Duration of study: Samples were bought from Roundab...
Isolation and Antibiogram of Staphylococcus aureus from Patients Admitted at Specialist Hospital Okitipupa, Ondo State, Nigeria
Aims: To isolate and determine the antimicrobial resistance and susceptibilities of Staphylococcus aureus from patients hospitalized at State Specialist Hospital, Okitipupa, Ondo State, Nigeria. Study Design: Prospectiv...
Isolation and Characterization of Phosphate Solubilizing Bacteria from the Rhizosphere of Faba Bean (Vicia faba L.) in Meknes Region, Morocco
Aims: The objective of this research was to isolate phosphate solubilizing bacteria (PSB) from faba bean rhizosphere in Meknes region and evaluate in vitro their potential for P solubilization as well as their response t...