Detection of Aspergillus flavus using PCR method from fungus infested food grains collected from local market
Journal Title: Annals of Plant Sciences - Year 2018, Vol 7, Issue 1
Abstract
India is an agrarian country two-thirds of its population is engaged directly or indirectly in agricultural activities. In recent years many food borne pathogens have become major threat to public health and safety. The consumption of contaminated food grains or products has been considered to be the leading source of human food borne infections. Surveillance studies have provided data and a better understanding into the existence and spread of food borne pathogens. Aflatoxins produced by Aspergillus species are important toxic secondary metabolites known for their impacts on animal and human health, and their effects on the economic loss of key grain and nut crops. Several molecular techniques (including multilocus sequence typing, pulsed field gel electrophoresis, DNA sequencing, multiplex PCR, RAPD, and many more) are available for detection and characterisation of pathogenic microorganisms from food samples, which provide reliable epidemiological data for tracing the source of infections. Present study highlights the possible use of PCR technique, in surveillance and detection of A. flavus in fungal infested food grains. The current study was carried out to elucidate the infestation of aflatoxin producing fungus on both kharif (groundnut, rice and maize) and Rabi crops (wheat, gram and soybean). Total 15 samples were collected randomly from local market of Gwalior (M.P). Out of fifteen only nine (60%) samples were found to be Aspergillus positive. Seven samples were infested by Aspergillus flavus and two by A. niger. The selected fungal isolates were identified by amplifying aflR gene of A. flavus in Thermo Cycler.
Authors and Affiliations
Renu Khare, Agarwal M. K. , Sameer S. Bhagayavant, Poonam Verma, Nagar D. P.
Keywords
Related Articles
- EP ID EP280518
- DOI 10.21746/aps.2018.7.2.16
- Views 111
- Downloads 0
Perpetuation of rice blast pathogen (Magnaporthe grisea) under temperate conditions of Kashmir, India.
Investigations were carried out during the months of October to March on various common specimens viz, paddy straw, stubbles, weed straw, soil, rice residue (husk), seeds and undecomposed organic material to ascertain th...
In-silico identification of cis- acting regulatory elements in 5’ regulatory region of Betaine aldehyde dehydrogenase isoenzymes of selected monocot and dicot
It’s challenging to understand the multifarious gene regulatory networks. Diverse biological processes, are mostly conferred by nature of cis-acting regulatory elements (CARE) located within the regulatory region which i...
Two new additions to the flora of Telangana, India.
Ipomoea triloba L. and Merremia dissecta (Jacq.) Hallier f. are reported here as an addition to the flora of Telangana. Detailed descriptions and photo plates are provided to facilitate its easy identification.
Impact of Erodium moschatum (L.) L.'Hér. extracts against some bacteria and fungi species
The effectiveness of Erodium moschatum extracts (ethanol and methanol, water) were tested against pathogenic bacteria (two gram positive: Staphylococcus aureus and Streptococcus lactis and two gram negative: Escherichia...
Allergic evaluation of Roystonea regia (Kunth.) O. F. Cook. among Keralites, India.
Aerobiological studies conducted in Kerala (India), revealed the incidence of pollen grains of Roystonea regia (Kunth.) O.F.Cook. belonging to the family Aracaceae as a common airborne pollen member. Previous clinical st...