Detection of Chloramphenicol Resistance Genes (cat) in Clinical Isolates of Pseudomonas aeruginosa with Polymerase Chain Reaction Method
Journal Title: Indonesian Journal of Clinical Pharmacy - Year 2014, Vol 3, Issue 4
Abstract
Pseudomonas aeruginosa is an opportunistic Gram negative bacteria, which may cause infection in eyes, ears, skin, bones, central nervous system, gastrointestinal tract, circulatory system, heart, respiratory system, and urinary tract. Recently, chloramphenicol is no longer used as the main option of the therapy due of its resistance case. The aim of this research was to detect the presence of gene which is responsible to chloramphenicol resistance in clinical isolates of P. aeruginosa. These bacteria isolated from pus of external otitis patients in Hasan Sadikin Hospital in Bandung City. Polymerase Chain Reaction (PCR) method (colony-PCR and DNA-PCR) were performed to detect this resistance gene. Electropherogram from PCR products showed that the chloramphenicol resistance in clinical isolates of P. aeruginosa was caused by cat gene (317 bp). Based on this research, cat gene may be used to detect the chloramphenicol resistance in patients with external ostitis.
Authors and Affiliations
Tiana Milanda, Lisa K. Dewi, Sri A. F. Kusuma
Keywords
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- EP ID EP352914
- DOI 10.15416/ijcp.2014.3.4.141
- Views 84
- Downloads 0
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