Detection of gonorrhea among HIV infected patients by polymerase chain reaction
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2015, Vol 5, Issue 7
Abstract
Objective: To determine the burden of gonorrhea among HIV patients using PCR technique. Methods: A cross sectional study was carried out in a HIV clinic of Sparsha Nepal from January to March, 2011. Standard microbiological procedures were followed during collection and processing of urine samples. DNA amplification, isolation and detection were carried out. Gonorrhoea diagnosis was done by multiplex polymerase chain reaction. Results: Among the 119 HIV positive patients, 12 (10.08%) were positive for N. gonorrheae. The cases were more among the males than in females. The age of patients ranged from 20- 45 years and the highest prevalence was among the age group 20-35 years. The distribution of gonorrhea infected patients according to marital status revealed that 8 cases were among the married patients. Conclusions: This study revealed that the incidence of gonorrhoea was higher in males and in married people. So, the control measures should be targeted to these groups of people.
Phytochemical and antimicrobial study of the seeds and leaves of Peganum harmala L. against urinary tract infection pathogens
Objective: To investigate the antibacterial effect of Peganum harmala (P. harmala) extracts against urinary tract infection pathogens isolated from infected patients. Methods: Agar diffusion technique was used for detect...
Developing ultra deformable vesicular transportation of a bioactive alkaloid in pursuit of vitiligo therapy
Objective: To develop transfersomal formulation integrated with piperine intended for vitiligo. Methods: Film hydration technique was employed in the preparation of transfersomes. Modified diffusion cell, consistency tes...
Efficacy of Stigmast-5-en-3β -ol Isolated from Salvadora persica L. as Antihyperlipidemic and Anti-tumor agent: Evidence from animal studies
Objective: To explore the anti-hyperlipidemic and anti-tumor effect of ethanolic extract, chloroform fraction and stigmast-5-en-3毬 -ol from stems of Salvadora persica L. Methods: The antihyperlipidemic effect of ethanol...
In vitro study on the inhibitory effect of ketamine to LPS-induced apoptosis in HUVEC culture
Objective: To determine the effect of lipopolysaccharide (LPS) exposure and treatment with ketamine to the levels of nitric oxide, intracellular calcium and caspase-3 of human umbilical vein endothelial cell culture. Met...
Modelling spatial relationship between climatic conditions and annual parasite incidence of malaria in southern part of Sistan&Balouchistan Province of Iran using spatial statistic models
Objective: To model spatial relationship between climatic conditions and annual parasite incidence (API) of malaria in southern part of Sistan&Balouchistan Province of Iran using spatial statistic models . Methods: A geo...