Detection of Hepatitis B Genotypes in HBsAg & HBeAg Carriers
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2018, Vol 7, Issue 5
Abstract
Objective: Hepatitis B is noteworthy medical issues that may include the late continuation of liver cirrhosis and hepatocellular carcinoma. The present study aimed for the detection and diffrentiation of Hepatitis B virus HBsAg inactive non-replicative carriers, HBeAg-positive inactive replicative carriers, active carriers & HBeAg-negative chronic hepatitis B by Real Time PCR and their genotyping Methods: This research conducted on 245 positive for HBsAg, 118 (48.16 %) were male and 127 (51.84%) were female patients, which was performed in central research station labortory of Microbiology at netaji subhash Chandra Bose subharti Medical College and Hospital, Meerut Between November 2016 to April 2018. The sera were separated and screened for HBsAg by ELISA kit. Positive samples for HBsAg were tested for HBeAg ELISA kit and DNA Viral load then sequenced for genotying Results: Of the 245 HBsAg Positive case 55 (1.12%) were HBeAg positive. In 16 PCR positive and HBV genotyping, In HBsAg inactive Non-Replicative 37.5% (n=6) genotype-B and 6.25% (n=1) genotype-A, In HBeAg inactive Replicative 12.5% (n=2) genotype-B and 12.5% (n=2) genotype-A and In HBeAg Active Chronic Hepatitis B 18.75% (n=3) genotype-B and 12.5% (n=2) genotype-A were detected Conclusions: Management strategy, using HBsAg, HBeAg and HBV DNA viral load, seems adequate for the confirmation and diffrentiation of Hepatitis B virus inactive, active carriers & HBeAg-negative chronic hepatitis B patients and genotype B was more prevalent in comparission to genotype A. Distribution of carriers & genotypes, help physicians to prescribe proper antiviral/interferon therapy according to current genotyping pattern in this region Keywords: Hepatitis B virus, Carrier State, HBsAg, HBeAg, RT-PCR
Authors and Affiliations
Khan Salman
Keywords
Related Articles
- EP ID EP487278
- DOI -
- Views 120
- Downloads 0
Comparative Study for the Assessment of Compliance in Alcoholics and Non-Alcoholic Patients on Anti-tubercular Therapy – A Prospective Observational Study
Background: Compliance to Anti-tubercular therapy is very important for the treatment and also to prevent the relapse or failure of the treatment. Nevertheless, many people fail to adhere to the therapy and various facto...
Knowledge towards digital Information and adverse drug reaction monitoring
Background: Pharmacy as a health profession has responsibility for ensuring the safe, effective and rational use of medicines. It's tough to get any medicine which is absolutely safe. Adverse drug reactions (ADRs) contri...
COMPARISION OF LEVELS OF PERIODONTOPATHIC BACTERIA BY POLYMERASE CHAIN REACTION IN CHRONIC AND AGGRESSIVE PERIODONTITIS PATIENTS BEFORE AND AFTER NON-SURGICAL THERAPY WITH SUBGINGIVAL ANTIMICROBIAL (BIOTENE) IRRIGATION.
INTRODUCTION: Periodontitis is an inflammatory disease of the supporting tissues of teeth caused by specific group of microorganisms resulting in destruction of periodontal ligament, alveolar bone with pocket formation...
EVALUATION OF ANTI-ULCER ACTIVITY OF CHLOROFORM AND ETHER EXTRACT OF JASMINUM OFFICINALE L. LEAF
Jasminum OfficinaleL. leaf had been collected form herbal garden of dravyagunavigyan, National Institute of Ayurveda and Botanical Authenticate Botanist of Institute of Biomedical and Industrial Research, the microscopic...
Effect of 50% ethanolic extract of roots of Cissampelos pareira on some bio-chemical parameters to investigate the hepato-protective study in animal model (Rats)
In phytotherapeutic approach, the emphasis is on the development of a new drug whose extraction and fractionation have emanated on the basis of therapeutic activity. Cissampelos pareira Linn. (C. microcarpa, De Candolle)...