Detection of mecA Gene Associated with Methicillin Resistant Staphylococcus aureus and its Alternatives using Nanoparticles and Chia Seeds
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2017, Vol 6, Issue 11
Abstract
Staphylococcus aureus, a member of the family Micrococcaceae, is a Gram-positive coccus whose cells tend to occur either singly or if dividing cells do not separate, form pairs, tetrads and distinctive irregular “grape-like†structures, which is resistant to few antibiotics like Methicillin which is termed as Methicillin resistant Staphylococcus aureus. MRSA was isolated from the pus sample. Confrmation of MRSA was done by using Kirby Bauer disk method. Followed by sub culturing in Luria bertani broth and DNA isolation was performed by phenol chloroform method and confrmed by AGE (Agarose gel electrophoresis). The amplifcation of mecA gene thermocycler-PCR was done. Restriction fragmented linked polymorphism was done for knowing how much restriction sites are available for organism. To calculate the molecular weight of the protein SDS-PAGE (Sodium dodecyl sulphate - Polyacrylamide gel electrophoresis) was performed. Followed by alternative measures was done, by using Silver nanoparticles at different concentrations and Chia seeds against MRSA.
Authors and Affiliations
M. Rajamani| Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore, Tamil Nadu, India, Corresponding e-mail: cbnrcindia@gmail.com, Jesteena Johney| Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore, Tamil Nadu, India, R. Ragunathan| Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore, Tamil Nadu, India
Keywords
Related Articles
- EP ID EP12490
- DOI -
- Views 423
- Downloads 25
Diagnostic dilemma in reckoning cytomegaloviral infection is prompting increased mortality among abandoned newborns of India
This article emphasizes on an almost untouched aspect of medical adversity concerning the health ordeal of the abandoned newborns in India. The case described here is that of an abandoned female newborn suffering from...
Role of Bronchoscopy in Malignant Pleural effusion
The aim of this study was to assess the role of Bronchoscopy in plural effusion in cancer condition. Pleural effusion is one of the commonest problems with which patients present to the hospital. Around a million patien...
Osteoporosis Knowledge and Beliefs Among Jordanian Men at Karak City
Aim: Osteoporosis is a skeletal disorder in which the bones become weak, brittle, and more likely to break. This disease is excessively prevalent among women, but it may also affect the male individuals. There is a lack...
EFFECT OF REPETITIVE MCKENZIE LUMBAR SPINE EXERCISES ON CARDIOVASCULAR SYSTEM
Background& Purpose:McKenzie exercises for the lumbar spine, which are done repeatedly, such as flexion in standing (FIS), extension in standing flexion in lying (FIL) & extension in lying (EIL) have been used in the m...
EFFECTIVENESS OF PEER-COUNSELING FOR PROMOTING OPTIMAL COMPLEMENTARY FEEDING PRACTICES AMONG INFANTS BELONGING TO URBAN SLUMS OF DELHI
Background: Promotion of appropriate complementary feeding practices is important in reducing malnutrition in infants and young children. Yet, India has dismal rates of optimal complementary feeding practices which are...