Detection of Metalo-Beta-Lactamase Gene in Carbapenem Resistant Pseudomonas Aeruginosa Isolated From Lahore, Pakistan
Journal Title: Pakistan Biomedical Journal - Year 2020, Vol 3, Issue 1
Abstract
Pseudomonas aeruginosa is a widespread organism, caused severe nosocomial infection in human and associated with multiple drug resistance (MDR) Objective: The present study was carried out to observe current antimicrobial resistant pattern of Pseudomonas aeruginosa in Lahore and to detect the Metallo-beta-lactamase (MBL) gene in carbapenem resistant Pseudomonas aeruginosa Methods: By screening 360 samples total 123 Pseudomonas aeruginosa was identified by standard microbiology techniques such as microscopy and biochemical testing. The isolated Pseudomonas aeruginosa was evaluated for drug resistance by disc diffusion method and polymerase chain reaction (PCR) was used to identify the carbapenem resistance causing gene (bla-VIM and bla-IMP) Results: Following antibiotic resistant pattern was observed, Gentamycin (59.00%), Ceftazidime (58.7%), Ceftriaxone (58.00%), Cefotazime (57.0%) and Ciprofloxacin (55.00%). Resistance rates to carbapenem group of antibiotics is Doripenem (30.5%) Meropenem (31.0%) and Imipenem (28.0%). Out of 123 samples of Pseudomonas aeruginosa, 28 isolates were found resistant to carbapenem group of antibiotic which was supposed to be highly sensitive for this bacterium. Molecular based identification of resistance genes showed that bla-IMP gene was present in 32.1% (09) and bla-VIM was found positive in 17.8% (04) samples. Metallo-beta-lactamasesproducing genes (bla-VIM and bla-IMP), amongcarbapenem resistant Pseudomonas aeruginosa were detectedin 28.1% of samples. If other carbapenem resistant gene were also included this number might be higher. Conclusions: PCR based test should be included in routine laboratory examination for quick detection of the resistance causing genes
Authors and Affiliations
Humera Kausar 1,2*, Shabbir Hussain2 and Afia Muhammad Akram 3
Keywords
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- EP ID EP694185
- DOI https://doi.org/10.52229/pbmj.v3i1.4
- Views 139
- Downloads 0
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