Detection of protozoans Babesia microti and [i]Toxoplasma gondii[/i] and their co-existence in ticks (Acari: Ixodida) collected in Tarnogórski district (Upper Silesia, Poland)
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2015, Vol 22, Issue 1
Abstract
[b]Introduction[/b]. Ticks (Acari: Ixodida) are vectors of many pathogens i. a: [i]Babesia microti [/i][Bm] and [i]Toxoplasma gondii[/i] [Tg]. In Poland, Ixodes ricinus [Ir] is the main vector of both pathogens. This tick species and pathogens transmitted by them are a significant threat to human and animal health. objectives of the study. Detection of the protozoans Bm and Tg in ticks collected in the Tarnogórski district area. [b]Material and methods[/b]. The ticks were collected from vegetation and pets in the spring period of their activity on the territory of the Tarnogórski district. The parasites were preserved in 70% ethanol. DNA was isolated by ammonia method. Bm was detected by nested-PCR using specific primers for the 18S rRNA sequence. To detect the Tg a commercial kit was used. The PCR products were separated on 2% ethidium bromide stained agarose gels and visualised under UV light. [b]Results[/b]. It was showed that all collected ticks belonged to the species Ir. Bm was detected in 50.87% and Tg in 64.91% of all examined ticks. Co-existence of these both protozoans in 36.84% of total examined ticks was noted. [b]Conclusions[/b]. The study showed a high risk of exposure to Bm and Tg in the studied area. Ticks Ir play an important role in the transmission of Bm in this region. Demonstrating a high percentage of ticks collected from animals infected with Tg may indicate their important role in the transmission of this pathogen, but it requires a further studies.
Authors and Affiliations
Marek Asman, Krzysztof Solarz, Piotr Cuber, Tomasz Gąsior, Ewa Szilman, Ewelina Tondaś, Anna Matzullok, Natalia Kusion, Karol Florek
Keywords
Related Articles
- EP ID EP80919
- DOI -
- Views 127
- Downloads 0
Common ophthalmic problems of urban and rural postmenopausal women in a population sample of Raciborz district, a RAC-OST-POL Study
Introduction and objective. We wished to establish the prevalence of eye diseases and eye disease risk factors at postmenopausal age and to compare ophthalmic problems in urban and rural areas of Raciborz.Patients and me...
Diverticular bleeding complicating dual antiplatelet therapy after drug-eluting stent implantation
Acute lower gastrointestinal bleeding is defined as a bleeding situation in which blood loss has been occurring for less than 3 days resulting in haemodynamic instability, anaemia, or the need for blood transfusion. Diver...
Mental symptoms among Finnish farm entrepreneurs.
The prevalence of mental symptoms among Finnish farm entrepreneurs in 2004 and 1994 was examined in two cross-sectional studies. The aim was also to examine associations between symptoms and background factors. Two rando...
Occupational accidents with mowing machines in Austrian agriculture
The number of recognized accidents during agricultural work is still very high in Austria. In the years 2008 to 2009, there occurred 84 approved work accidents with mowing machines. The main causes of accidents were the...
Metabolic syndrome in Poland – the PONS Study
Introduction: In Central and Eastern European countries, cardiovascular disorders (CVD) in middle age are much more common than in Western Europe, and it is imperative to understand the causes underlying this excess dise...