Detection of RNA1 and RNA2 of Soil-borne wheat mosaic virus in winter wheat grown from infected seeds
Journal Title: Journal of Plant Protection Research - Year 2016, Vol 56, Issue 4
Abstract
A Polish isolate of Soil-borne wheat mosaic virus (SBWMV-Pol1) was characterized by limited pathogenicity and a low concentration of virus particles in infected plant tissues. The aim of this research was to consider the possibility of seed-transmission dissemination of the virus. Seeds of winter wheat cv. Muszelka served as material for the studies. Two methods were involved in the diagnostics of seedlings grown from potentially infected seeds: enzyme-linked immunosorbent assay (ELISA), as the screening assay and immuno-capture-reverse transcription-polymerase chain reaction (IC-RT-PCR) for molecular confirmation of the infection. RNA1 and RNA2 of SBWMV-Pol1 were detected in 6 out of 1,410 plants submitted to diagnostic procedures. The possibility of seed transmission of SBWMV-Pol1 was discussed.
Authors and Affiliations
Małgorzata Jeżewska, Katarzyna Trzmiel, Aleksandra Zarzyńska-Nowak
Keywords
Related Articles
- EP ID EP184605
- DOI 10.1515/jppr-2016-0057
- Views 82
- Downloads 0
Symbiotic association between golden berry (Physalis peruviana) and arbuscular mycorrhizal fungi in heavy metal-contaminated soil
Physalis peruviana is one of the most promising tropical fruit plants because of its rapid growth, high yield, and nutritional quality. This study was designed to investigate plant development under heavy metal contamina...
Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants
The tuber necrotic strain of Potato virus Y (PVYNTN) causes widespread disease and has severe negative effects on the growth and yields of plants, especially those of the Solanaceae family. The consequences of residual t...
Developing an artificial diet for rearing Orius albidipennis Reuter (Het., Anthocoridae)
The use of suitable mass rearing methods is crucial to establish successful inundative or inoculative biological control programs. The development of an artificial diet considerably reduces costs of mass rearing. In this...
Oviposition deterrent and ovicidal properties of Calotropis gigantea (L.) leaf extract to Paraeucosmetus pallicornis (Dallas) in rice
Paraeucosmetus pallicornis (Dallas) is a new pest in Indonesia which decreases rice production and quality. This pest causes the grains to become flat, hollow, brownish, break easily when milled, and bitter. This researc...
Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: the use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
Fungal entomopathogens can naturally regulate populations of various insects. The entomopathogen Beauveria bassiana (Bals.-Criv.) Vuill. is also able to endophytically colonize different plants. Endophytic colonization b...