Detection of the Bovine Leptin Gene rs29004488 Polymorphism by Introducing a New Restriction Enzyme Site For PCR-RFLP

Journal Title: MAS Journal of Applied Sciences - Year 2023, Vol 8, Issue 5

Abstract

Leptin is a hormone mainly synthesized in adipose tissue and plays an important role in the regulation of appetite, energy metabolism, body weight and reproductive functions. The rs29004488 polymorphism in the 2nd exon of the bovine leptin gene, which results in an amino acid change from arginine to cysteine, has been reported to be associated with economically important traits such as growth, carcass traits, milk yield and reproduction. In cattle, the leptin gene rs29004488 polymorphism can be detected by PCR-RFLP method using Kpn2I enzyme. However, this method has some limitations in terms of primer degradation. In this study, a new PCR-RFLP method was investigated to detect rs29004488 polymorphism of leptin gene in cattle. Using the created restriction site PCR (CRS-PCR) method, PstI enzyme recognition site was introduced into the PCR products, and rs29004488 polymorphism was successfully detected using newly designed primers and PstI enzyme. The obtained results were confirmed by DNA sequence analysis and comparison with Kpn2I enzyme cleavage results. This study demonstrates that the rs29004488 polymorphism can be detected by an efficient and low-cost method using CRS-PCR-RFLP and PstI enzyme.

Authors and Affiliations

Murad GURSES, Metin BAYRAKTAR, Huseyin YUCE, Ebru ONALAN

Keywords

Related Articles

Determination and Prevalence of Some Important Pathogens in Newborn Calf Diarrhea in Bingol and its Surroundings

The aim of this study was to determine clinically the prevalence of the most common pathogens causing diarrhea in 120 calves with diarrhea from 0 to 30 days old by rapid diagnostic test (HTT), standard diagnostic methods...

The Concerns That New Technology Brings To The Agriculture And Food Sector

Nanotechnology has already taken its place with its widespread applications in various industries. It is seen that the food and agricultural sectors also share in the commercial expansion of nanomaterials. Nanomaterials...

The Relationship Between Mindfulness, Rumination and Depression

Today, the concept of Mindfulness is becoming popular and frequently used in clinical practice and in the lives of individuals. Mindfulness is a skill that can be developed over time, as we experience it, and it is a con...

Determination of Yield and Quality Properties of Different Flax (Linum usitatissimum L.) Genotypes in Eskisehir Ecological Conditions

This research was conducted to determine the performance of different flax (Linum usitatissimum L.) genotypes (Sari-85, Clli 1351, Clli 1370, Clli 1400, Clli 1412, Clli 1423, Larnaka, Milas, NewTurk and Dillman) in terms...

Using Waste Papers in the Field of Art by Making it Functional: Kocaeli Province Example

Paper production and consumption in our country has increased rapidly especially in recent years. The use of waste paper in paper production is increasing every year due to population growth, industrialization and urbani...

Download PDF file
  • EP ID EP723754
  • DOI 10.5281/zenodo.10024106
  • Views 40
  • Downloads 0

How To Cite

Murad GURSES, Metin BAYRAKTAR, Huseyin YUCE, Ebru ONALAN (2023). Detection of the Bovine Leptin Gene rs29004488 Polymorphism by Introducing a New Restriction Enzyme Site For PCR-RFLP. MAS Journal of Applied Sciences, 8(5), -. https://europub.co.uk./articles/-A-723754