DETERMINATION OF CLINDAMYCIN PHOSPHATE IN DIFFERENT VAGINAL GEL FORMULATIONS BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Journal Title: Acta Facultatis Medicae Naissensis - Year 2013, Vol 30, Issue 0
Abstract
Clindamycin is an antibiotic effective against Gram-positive aerobes and both Gram-negative and Gram-positive anaerobic pathogens. Clindamycin (its phosphate ester) was used as a model drug for our study in order to check the efficacy of the developed vaginal gel formulations. The aim of this study was to develop an analytical method suitable for the determination of clindamycin phosphate in three different vaginal gel formulations. The main difference between the three formulations was the use of different gelation agents in the formulations (Carbopol® 940, Carbopol® Ultrez 10 and Pemulen® TR 1). A new isocratic high performance liquid chromatography method based on reverse phase separation has been developed for the determination of clindamycin phosphate. The separation was achieved on a Zorbax Eclipse plus C8 column with a mobile phase of acetonitrile and pH 2.5 phosphate buffer and UV detection at 210 nm. A linear response (r>0.99) was observed in the range of 10.0-80.0 µg mL-1. The method shows good recoveries and relative standard deviations were less than 1.0%. The method can be applied to assess the stability of clindamycin phosphate in vaginal gel formulations. It can be used for quality control and stability study samples analysis. Clindamycin phosphate concentration was different in vaginal gel formulations with different gelation agents, so the conclusion is that gelation agents (gel carriers) have a significant influence on the active drug concentration. The obtained results confirm that vaginal gel formulation with Carbopol® 940 shows the maximal stability during the analysis and provides the optimal value of clindamycin phosphate concentration.
Authors and Affiliations
Milica Stanković, Vesna Savić, Valentina Marinković
Keywords
Related Articles
- EP ID EP157482
- DOI 10.2478/v10283-012-0038-y
- Views 90
- Downloads 0
BRONHOSKOPIJA I BRONHOALVEOLARNA LAVAŽA KOD DECE SA INFEKCIJOM DONJIH DISAJNIH PUTEVA I NAJČEŠĆE IZOLOVANIM PATOGENIM MIKROORGANIZMIMA
Ova studija predstavlja pregled najčešćih patoloških mikroorganizama koji su uzročnici plućnih infiltrata, izolovanih u bronhoalveolarnom lavatu (BAL) pedijatrijskih bolesnika. Cilj rada bio je da prikaže rezultate bronh...
Evaluation of the Influence of Treatment Exposure on Neuropeptide Leptin Hormone and Obesity Risk among Jordanian Breast Cancer Women
Breast cancer (BC) is the most prevalent and life-threatening malignant tumor in adult females. Little is known about the association between neuropeptide leptin hormone and development of BC. The aim of the paper was to...
Treatment of Patients with Risk Factors: Compliance and Adherence - - - Lečenje bolesnika sa faktorima rizika: komplijansa i adherenca
Hypertension and dyslipidemias represent important risk factors for the occurrence of cardiovascular diseases which have a high mortality rate. Although we know how to treat both, the success rate remains low. More than...
ANTIBIOTICI U TERAPIJI PARODONTALNOG OBOLJENJA
Sistemski antibiotici se učestalo koriste u terapiji parodontalnih infekcija. Dok se ovi lekovi uglavnom koriste na empirijskoj osnovi, neki lekari se bore za to da, racionalna upotreba antibiotika treba biti normatirana...
TELEKONSULTACIJA U STOMATOLOGIJI PREKO XPA3 ONLINE SISTEMA: PRIKAZ SLUČAJA
Telemedicina predstavlja interaktivnu audiovizuelnu komunikaciju između lekara i pacijenata. Telemedicinski centar u Kosovskoj Mitrovici organizuje i upravlja procesom telekonsultacije koristeći kompjutersko-medicinski s...