Determination of esters and amides of aminobenzoic acid in the composition of finished dosage forms with acidic monoazo-dye tropaeolin O
Journal Title: Фармацевтичний журнал - Year 2015, Vol 2, Issue 2
Abstract
Esters of aminobenzoic acid are widely used in medical practice as effective painkillers of local action, and its amide as is an antiarhythmic drug. The peculiarity of these drugs, in terms of analytical control, is a low content of active ingredients. It requires sufficiently sensitive methods for their quality control. Usually in these cases the HPLC method are used, which are relatively expensive and demand quite high qualification of an operator; while the spectrophotometric method is much cheaper, more accessible, more rapid, and moreover, it can be used as a routine method in analytical laboratories. The aim of this work was to study the interaction of p-aminobenzoic acid and its amide (procainamide), three esters (benzocaine, procaine, oxybuprocaine) and one ester of m-aminobenzoic acid (proxymetacaine) with azo-dye tropaeoline O to develop new spectrophotometric method of their determination in various dosage forms with satisfactory validation parameters. It was established that diazo salts of primary aromatic amines react with tropaeoline O to form the coloured product with the maximum absorbance at 595 nm, while the dye does not absorb the light at this wavelength. The conditions of diazo salts amines interaction with azo dye are as follows: the reaction takes place in the medium of 0,01 M sodium tetraborate at pH 10–11 at 2-fold excess of tropaeoline O. Also the method of these amines spectrophotometric determination has been elaborated. The method possesses a wide linearity range, viz. from 0,4 to 20,0 mg/ml depending on the detectable amine. The content of procaine, procainamide, oxybuprocaine and proxymetacaine in pharmaceutical medicines was determined. The results are consistent with the content of amines by the manufacturer, as well as with the results obtained by nitritometric titration.
Authors and Affiliations
M. Ya. Smolinska, I. Ya. Kotsiumbas, G. Y. Teslyar, M. V. Yurkevych, M. B. Smeyko
Automation of electronic system for the formation of bibliography of scientific works in higher medical educational establishments of Ukraine
Establishments, where workers accomplish scientific investigation, give information in reporting data about conducted research in qualitative and quantitative forms, indicating references to published results of investig...
The main optical characteristics of UV-spectra of 5-(adamantane-1-yl)-4R-1,2,4-triazole-3-thiones derivatives
The development of pharmaceutical chemistry confronts molecular spectroscopy the problem of calculating the electronic states, assumptions and explanations of the various properties of complex organic compounds. In recen...
Synthesis of some new thiazolo[4,5-b]pyridin-2-ones and research of their anti-inflammatory activity
In modern theoretical and clinical medicine inflammation problem remains one of the main.Deregulation of inflammatory processes leads to specific pathologies.There is a significant amount of drugs used to treat inflammat...
Histological study of the drug PEG-Filstim sub-acute toxicity
One of the critical steps in development of safe and efficient drugs during their pre-clinical trials are toxicity studies. Therefore, the aim of our work was to study PEG-Filstim toxic effects on animal internal organs...
Study on domestic market of medicinal products of plant origin
The popularity of herbal remedies and medicines, with the participation of biologically active substances from plants is traditionally high. The annual increase of the domestic market for herbal remedies is due to the pr...