DETERMINATION OF HYDROXYPROLINE IN COLLAGEN BIOMATERIALS FOR MEDICAL USE AND VALIDATION OF METHOD
Journal Title: Revista de Pielărie Încălţăminte - Year 2016, Vol 16, Issue 2
Abstract
The paper presents a method for determining hydroxyproline in collagen biomaterials for medical use produced in The Collagen Department of INCDTP – Division ICPI and its validation. The method has two stages: the first one consists in hydrolysis of proteins in the sample of protein product to the form of amino acids, among which hydroxyproline. The second stage refers to selective highlighting of hydroxyproline using a specific colour reaction and quantitative assessment against a reference standard with known concentration. The method was validated to establish performance parameter and to check compliance with the set goal.
Authors and Affiliations
Gabriela MACOVESCU, Ciprian CHELARU, Mădălina Georgiana ALBU KAYA, Luminiţa ALBU
Keywords
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