Determination of nicotine levels in the leaves of some Nicotiana tabacum varieties cultivated in Syria
Journal Title: Herba Polonica - Year 2015, Vol 61, Issue 4
Abstract
Introduction: Tobacco is the most widely grown non-food crop in the world. Nicotine is the most abundant volatile alkaloid in tobacco leaves. Objectives: This work aimed at measuring nicotine levels in the leaves of seven different varieties of Nicotiana tabacum, namely: Virginia, Burlip, Katrina, Shk al-bent, Zegrin, Basma and Burley, cultivated in Syria. Methods: Nicotine was extracted according to approved method and its concentration was determined by LC/MS/MS in comparison with a standard material dilution series. The percentage of nicotine concentration was calculated manually. Statistical analysis was used to assess the significance of differences among variables and to perform multiple comparisons. Results: The amount of nicotine in dry weight of tobacco leaves represented 6.7% in Virginia variety, 4.9% in Burlip, 4.84% in Katrina, 4.67% in Shk al-bent, 4% in Zegrin, 3.3% in Basma and <3% in Burley. Significant differences in nicotine concentration were found among the different varieties as determined by LSD test at a level of 0.05. Conclusion: This study shows the importance of tobacco varieties grown in Syria, particularly Virginia, Burlip, Katrina, as a cheap and wealthy source for nicotine to be used in some industries.
Authors and Affiliations
Ghaleb Tayoub, Huda Sulaiman, Malik Alorfi
Chemical diversity within strawberry (Fragaria vesca L.) species
The chemical diversity within wild strawberry (Fragaria vesca L.) species was studied depending on its origin (natural vs cultivated). The herb was collected at the full flowering stage. Following items were determined...
Impact of Panax ginseng and Ginkgo biloba extracts on expression level of transcriptional factors and xenobiotic-metabolizing cytochrome P450 enzymes
Introduction: Despite widespread use of Panax ginseng and Ginkgo biloba, the data on the safety as well as herb-drug interactions are very limited. Therefore, we postulate that P. ginseng and G. biloba may modulate the a...
Maidenhair tree (Ginkgo Bilobae) leaf extraction products in the light of Biopharmaceutics Classification System (BCS) requirements and medium of diversified polarity (εM)
Model maidenhair tree (Ginkgo bilobae) leaf extracts were created basing on medium of diversified polarity (ԐM). Chromatographic analysis was performed with the HPLC method, with the so-called dry residue remaining after...
Effect of Nyctanthes arbor tristis leaf extract against scopolamine-induced cognitive impairment in rats
Nyctanthes arbor tristis (NATE) ethanol extract (150 mg/kg, orally) was evaluated for its protective effect against scopolamine-induced (1 mg/kg i.p.) cognitive impairments in rats using behavioral models like radial arm...
Antidiarrheal activity of fractions from aqueous extract of Detarium senegalense
The aim of this study was to evaluate the effect of different fractions of the aqueous crude extract of Detarium senegalense stem bark on castor oil-induced diarrhea. Castor oil-induced diarrhea, gastrointestinal motilit...