Determination of Optimum Composition of Fermentation Medium for Citric Acid Production from Watermelon Rinds using Response Surface Methodology
Journal Title: Tropical Journal of Natural Product Research - Year 2017, Vol 1, Issue 1
Abstract
A four variable Box-Behnken design (BBD) coupled with response surface methodology (RSM) was employed to optimise the composition of fermentation medium for the production of citric acid from watermelon rind using Aspergillus niger in solid state fermentation (SSF). The four variables studied were glucose (C6H12O6), diammonium hydrogen phosphate ((NH4)2HPO4), potassium dihydrogen phosphate (KH2PO4) and sodium chloride (NaCl). Statistical analysis of the results showed that all the components of the medium ((NH4)2HPO4, KH2PO4 and NaCl) apart from glucose had a significant effect on citric acid production (p<0.05). A statistical quadratic model was developed by applying regression analysis to the experimental data. The model was statistically significant (p<0.0001) and showed a good fit with the experimental data. Citric acid production was positively influenced by high levels of glucose and NaCl while the reverse was the case for KH2PO4 and (NH4)2HPO4. The optimised nutrient medium contained glucose, (NH4)2HPO4, KH2PO4 and NaCl in proportions of 23.40 g/L, 0.19 g/L, 0.19 g/L and 0.59 g/L respectively. In validating the model, no significant difference was observed between predicted and experimental results. This study has demonstrated the feasibility of producing citric acid from watermelon rinds in SSF
Authors and Affiliations
Andrew Amenaghawon
Keywords
Related Articles
- EP ID EP349403
- DOI 10.26538/tjnpr/v1i1.3
- Views 82
- Downloads 0
Pyrenacantha staudtii Hutch and Dalz (Icacinaceae): A review of Phytochemistry, Pharmacology and Future Perspectives
Various parts (stem bark, leaves and roots) of Pyrenacantha staudtii Hutch and Dalz, Icacinaceae are used traditionally in Nigeria and other West and Central African folk medicine in the treatment of various ailments. Da...
Determination of Optimum Composition of Fermentation Medium for Citric Acid Production from Watermelon Rinds using Response Surface Methodology
A four variable Box-Behnken design (BBD) coupled with response surface methodology (RSM) was employed to optimise the composition of fermentation medium for the production of citric acid from watermelon rind using Asperg...
Characterization and Evaluation of the Suspending Potentials of Corchorus olitorius Mucilage in Pharmaceutical Suspensions
The study was aimed at extraction, characterization and evaluation of suspending potentials of Corchorus olitorius mucilage (COM) in comparison with acacia BP (ACG) in pharmaceutical suspensions. Corchorus olitorius muci...
Antiglycation and Antioxidant Activity of Funtumia africana Root Used in the Management of Diabetes
Received 04 April 2018 Revised 07 May 2018 Accepted 08 May 2018 Published online 10 May 2018 Funtumia africana (Benth.) Stapf (Apocynaceae) is used folklorically for the treatment of various ailments such as dysentery,...
Nigerian Medicinal Plants with Potential for the Treatment of Neglected Tropical Disease: Onchocerciasis
Received 20 June 2018 Revised 30 June 2018 Accepted 02 July 2018 Published online 08 July 2018 The present study is a survey of medicinal plants used to manage onchocerciasis in Ibarapa Local Government Area of Oyo Stat...