“Determination of potential virulence factors in clinical isolates of various Candida species to assess its clinical implication in a tertiary care hospital in Eastern India”.
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 7
Abstract
Candidiasis is the commonest fungal disease in human caused by various species of the yeast like fungi belonging to the genus Candida. It is commonly encountered as an endogenous infection due to its commensal nature. Transition from commensal to a pathogen is attributable to complex array of different virulence factors which directly interacts and damages mammalian host cells. Aim: The present study was intended to determine different virulence factors expressed by clinical isolates of Candida species and to consider its clinical implication which will help in providing rational and justified therapy to the patients. Methods and Material: 240 clinical isolates of Candida species obtained from various clinical specimens like nail (86), blood (44), deep tracheal aspirate (DTA) & bronchoalveolar lavage (BAL) (28), oral swabs & buccal washings (36), urine (30), corneal ulcer (10) and ear discharge (06) were included in the study and processed for speciation following standard protocol. The virulence factors detected were phospholipase activity in egg yolk agar, haemolysin production in Sabouraud’s Dextrose Agar (SDA) supplemented with blood, esterase activity in Tween 80 media, adherence assay and production of biofilm. Results: Among 240 Candida isolates, 80 were C.albicans, 60 C.tropicalis, 44 C.parapsilosis, 24 C.lusitanae, 16 C.glabrata, 12 C.krusei, and 04(four) C.stellatoidea. 118 (49.1%) isolates showed haemolysin production, 106 (44.1%) expressed phospholipase, 76 (31.6%) expressed esterase, 94 (39.1%) strains gave positive result in adherence assay and 116 (48.3%) Candida isolates were found to be biofilm producers. Conclusions: The above study emphasizes on detecting virulence traits of Candida isolates obtained from clinical samples as contaminant commensal Candida strains are not infrequent isolation from clinical samples.
Authors and Affiliations
Dr. Reena Ray (Ghosh), Dr. Mallika Ghosh, Dr. Nibedita Chatterjee, Prof. (Dr,) Mitali Chatterjee
Keywords
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- EP ID EP377432
- DOI 10.9790/0853-1707095661.
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