Determination of six arsenic species in human urine by HPLC-ICP-MS
Journal Title: Shanghai Journal of Preventive Medicine - Year 2023, Vol 35, Issue 2
Abstract
ObjectiveTo establish an High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry ï¼HPLC-ICP-MSï¼ method for determination of six arsenic species in human urineï¼including arseniccholine ï¼AsCï¼ï¼ arsenobetaine ï¼AsBï¼ï¼ arsenite ï¼As3+ï¼ï¼ dimethylarsinic acid ï¼DMA5+ï¼ï¼ monomethylarsonic acid ï¼MMA5+ï¼ï¼ and arsenate ï¼As5+ï¼.MethodsThe pH value of mobile phase and the content of anhydrous ethanol were optimized. Ammonium carbonate ï¼50 mmol·L-1ï¼ containing 2% anhydrous ethanolï¼ pH-8.5ï¼ mobile phase was selected. Cl- interference was eliminated by He mode. The arsenic species in 10-fold diluted human urine samples were separated by an Hamilton PRP X-100 anionic column. A method for the determination of six arsenic species was established.ResultsSix arsenic species could be separated in 13 minutes. The linear correlation coefficients were above 0.999. The limits of detection were 0.10â0.20 μg·L-1ï¼ and the limits of quantification were 0.30â0.50 μg·L-1. Precision experiments showed that RSD ranged from 5.96% to 9.07% when adding concentration 0.20 μg·L-1ï¼ from 2.48% to 6.38% when adding concentration 2.00 μg·L-1ï¼ and from 1.41% to 2.57% when adding concentration 5.00 μg·L-1. Accuracy test showed that the recoveries were 80%â125%.ConclusionThe established HPLC-ICP-MS method for determination of six arsenic species in human urine is rapidï¼ accurate and sensitive. It can be applied to the determination of arsenic species in human urine.
Authors and Affiliations
HE Yi,LIU Lanxia,XUE Liming,XIONG Libei,FANG Yamin,
Keywords
Related Articles
- EP ID EP742076
- DOI -
- Views 33
- Downloads 0
Current status of hand hygiene practices among primary healthcare workers and its influencing factors
Objective To investigate the current status and influencing factors of hand hygiene practices among primary healthcare workers， and to provide a reference for improving hand hygiene behavior in primary healthcare instit...
Social support and parent-child relationship among adolescents and their influence on emotions
ObjectiveTo explore the association between social supportï¼ parent-child relationshipsï¼ and depressionï¼ anxietyï¼ and loneliness among adolescentsï¼ in order to provide a reference for improving mental health in...
Progress in the study of the association between pubertal timing and blood pressure
With the increasing burden of hypertension in Chinese adults, the detection rate of hypertension among children and adolescents continued to rise, drawing increasing attention to the impact of pubertal timing on blood pr...
Major mechanisms of hepatitis B virus induced hepatocarcinogenesis
The occurrence and development of hepatocellular carcinoma ï¼HCCï¼ induced by chronic infection of hepatitis B virus ï¼HBVï¼ is a typical process of Cancer Evolution-development. Viral replicationï¼ viral mutationï¼...
Overview of the establishment of Shanghai twin birth cohort
Objective To establish the Shanghai twin birth cohort （STBC） and analyze the effects of genetic factors， shared environment， and non-shared environment interactions on birth health and growth and development of newbo...